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Systematic subcellular localization of novel proteins identified by large‐scale cDNA sequencing
Open accessAbstract
As a first step towards a more comprehensive functional characterization of cDNAs than bioinformatic analysis, which can only make functional predictions for about half of the cDNAs sequenced, we have developed and tested a strategy that allows their systematic and fast subcellular localization. We have used a novel cloning technology to rapidly generate N‐ and C‐terminal green fluorescent protein fusions of cDNAs to examine the intracellular localizations of >100 expressed fusion proteins in living cells. The entire analysis is suitable for automation, which will be important for scaling up throughput. For >80% of these new proteins a clear intracellular localization to known structures or organelles could be determined. For the cDNAs where bioinformatic analyses were able to predict possible identities, the localization was able to support these predictions in 75% of cases. For those cDNAs where no homologies could be predicted, the localization data represent the first information.
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Cited by
A subcellular map of the human proteome
Thul, P. J., Åkesson, L., Wiking, M., Mahdessian, D., Geladaki, A., Ait Blal, H., Alm, T., Asplund, A., Björk, L., Breckels, L. M., et al. (2017) · Science · doi:10.1126/science.aal3321 · Cited by 2,813
McDonnell, L. A., & Heeren, R. M. A. (2007) · Mass Spectrometry Reviews · doi:10.1002/mas.20124 · Cited by 1,082
Characterization of the CLEAR network reveals an integrated control of cellular clearance pathways
Palmieri, M., Impey, S., Kang, H., di Ronza, A., Pelz, C., Sardiello, M., & Ballabio, A. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddr306 · Cited by 1,022
COMPARTMENTS: unification and visualization of protein subcellular localization evidence
Binder, J. X., Pletscher-Frankild, S., Tsafou, K., Stolte, C., O'Donoghue, S. I., Schneider, R., & Jensen, L. J. (2014) · Database · doi:10.1093/database/bau012 · Cited by 668
MiD49 and MiD51, new components of the mitochondrial fission machinery
Palmer, C. S., Osellame, L. D., Laine, D., Koutsopoulos, O. S., Frazier, A. E., & Ryan, M. T. (2011) · The EMBO Reports · doi:10.1038/embor.2011.54 · Cited by 649
Metadata
- Authors
- Simpson, J. C., Wellenreuther, R., Poustka, A., Pepperkok, R., & Wiemann, S.
- Venue
- EMBO reports
- Published
- 2000-09
- Volume
- 1
- Issue
- 3
- Pages
- 287-292
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- OpenAlex
- W2149198894
- Cited by
- 354
- References
- 0
- FWCI
- 11.18
- Type
- journal article
- Language
- en
- Open access
- green
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- Simpson, J. C., Wellenreuther, R., Poustka, A., Pepperkok, R., & Wiemann, S. (2000). Systematic subcellular localization of novel proteins identified by large‐scale cDNA sequencing. EMBO reports. https://doi.org/10.1093/embo-reports/kvd058
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