The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
TRF1 is a dimer and bends telomeric DNA
Open accessAbstract
TRF1 is a mammalian telomeric protein that binds to the duplex array of TTAGGG repeats at chromosome ends. TRF1 has homology to the DNA-binding domain of the Myb family of transcription factors but, unlike most Myb-related proteins, TRF1 carries one rather than multiple Myb-type DNA-binding motifs. Here we show that TRF1 binds DNA as a dimer using a large conserved domain near the N-terminus of the protein for TRF1-TRF1 interactions. Dimerization was observed both in a complex with DNA and in the yeast two-hybrid assay. TRF1 dimers were found to require both Myb repeats for the formation of a stable complex with DNA, indicating a parallel between the DNA-binding mode of TRF1 and other Myb-related proteins. TRF1 was found to have a number of biochemical similarities to Rap1p, a distantly related DNA-binding protein that functions at telomeres in yeast. Rap1p and TRF1 both require two Myb motifs for DNA binding and both factors bind along their cognate telomeric sequences without showing strong cooperative interactions between adjacent proteins. Furthermore, TRF1 was found to bend its telomeric site to an angle of -120 degrees. Since Rap1p similarly distorts telomeric DNA, we propose that DNA bending is important for the function of telomeres in yeast and mammals.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“TRF1 has the ability to induce bending, looping, and pairing of duplex telomeric DNA (5, 6, 20, 21), activities that could facilitate the folding back of the telomere.”
From Control of Human Telomere Length by TRF1 and TRF2 (2000) · cites this paper as [5]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A novel genetic system to detect protein–protein interactions
Fields, S., & Song, O.-K. (1989) · Nature · doi:10.1038/340245a0 · Cited by 6,152
Moyzis, R. K., Buckingham, J. M., Cram, L. S., Dani, M. A. C., Deaven, L. L., Jones, M. D., Meyne, J., Ratliff, R. L., & Wu, J. R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.18.6622 · Cited by 2,326
Counter, C. M., Avilion, A. A., LeFeuvre, C. E., Stewart, N. G., Greider, C. W., Harley, C. B., & Bacchetti, S. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05245.x · Cited by 2,096 · cited 2×
“clock controlling cell senescence (Counter et al., 1992; reviewed in Harley, 1995) and as a step in tumorigenesis Since DNA fragments with three, six or 12 telomeric”
BackgroundMorin, G. B. (1989) · Cell · doi:10.1016/0092-8674(89)90035-4 · Cited by 1,495
The locus of sequence-directed and protein-induced DNA bending
Wu, H., & Crothers, D. M. (1984) · Nature · doi:10.1038/308509a0 · Cited by 1,454
Control of telomere length by the human telomeric protein TRF1
van Steensel, B., & de Lange, T. (1997) · Nature · doi:10.1038/385740a0 · Cited by 1,219
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
Guarente, L. P. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)01013-7 · Cited by 1,022
A mutant with a defect in telomere elongation leads to senescence in yeast
Lundblad, V., & Szostak, J. W. (1989) · Cell · doi:10.1016/0092-8674(89)90132-3 · Cited by 931
Telomeres: Beginning to Understand the End
Zakian, V. A. (1995) · Science · doi:10.1126/science.270.5242.1601 · Cited by 859
Structure and variability of human chromosome ends.
de Lange, T., Shiue, L., Myers, R. M., Cox, D. R., Naylor, S. L., Killery, A. M., & Varmus, H. E. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.2.518 · Cited by 848
Research works printed but not linked: 4
- Blackburn EH (1993) Telomerase. In Gesteland,R.F. and Atkins,J.F. (eds), The RNA World. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 557–576.
- Cooper J, Nimmo E, Allshire R and Cech T (1997) A Myb‐domain protein regulates telomere length and function in fission yeast. Nature, in press.
- de Lange T (1995) Telomere dynamics and genome instability in human cancer. In Blackburn,E.H. and Greider,C.W. (eds), Telomeres. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 265–295.
- Harley CB (1995) Telomeres and aging. In Blackburn,E.H. and Greider,C.W. (eds), Telomeres. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 247–265.
Cited by
Sorted by citations
Shelterin: the protein complex that shapes and safeguards human telomeres
de Lange, T. (2005) · Genes & Development · doi:10.1101/gad.1346005 · Cited by 3,056
Mammalian Telomeres End in a Large Duplex Loop
Griffith, J. D., Comeau, L., Rosenfield, S., Stansel, R. M., Bianchi, A., Moss, H., & de Lange, T. (1999) · Cell · doi:10.1016/s0092-8674(00)80760-6 · Cited by 2,447
Self-citationSwitching and Signaling at the Telomere
Blackburn, E. H. (2001) · Cell · doi:10.1016/s0092-8674(01)00492-5 · Cited by 2,139
How Shelterin Protects Mammalian Telomeres
Palm, W., & de Lange, T. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.41.110306.130350 · Cited by 1,802
TRF2 Protects Human Telomeres from End-to-End Fusions
van Steensel, B., Smogorzewska, A., & de Lange, T. (1998) · Cell · doi:10.1016/s0092-8674(00)80932-0 · Cited by 1,709
Metadata
- Authors
- Bianchi, A., Smith, S., Chong, L., Elias, P., & de Lange, T.
- Affiliations
- Rockefeller University (US)
- ORCID
- Bianchi, Alessandro
- Venue
- The EMBO Journal
- Published
- 1997-04-01
- Volume
- 16
- Issue
- 7
- Pages
- 1785-1794
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC1169781
- OpenAlex
- W2127440297
- Cited by
- 358
- References
- 67
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 11 · 2023: 10 · 2022: 6 · 2021: 16 · 2020: 3 · 2019: 13 · 2018: 5 · 2017: 14 · 2016: 8 · 2015: 9 · 2014: 17 · 2013: 12 · 2012: 13
- FWCI
- 8.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- University of North Carolina at Chapel Hill
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- TRF1, telomeres, protein dimerization, DNA bending, TTAGGG repeats, Rap1p, telomeric DNA binding, telomere function, protein-DNA complex
- MeSH terms
- Shelterin Complex, Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Cell Line, DNA, DNA-Binding Proteins, Mammals, Nucleic Acid Conformation, Recombinant Proteins, Repetitive Sequences, Nucleic Acid, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Transfection, Protein Biosynthesis, Polymerase Chain Reaction, Telomere, Conserved Sequence, Spodoptera, Dimerization, Saccharomyces cerevisiae Proteins, Telomere-Binding Proteins, Telomeric Repeat Binding Protein 1, Macromolecular Substances
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
