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Apg10p, a novel protein‐conjugating enzyme essential for autophagy in yeast

Shintani, Takahiro, Mizushima, Noboru, Ogawa, Yoko, Matsuura, Akira, Noda, Takeshi, Ohsumi, Yoshinori·1999·The EMBO Journal
DOI 10.1093/emboj/18.19.5234
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Abstract

Autophagy is a cellular process for bulk degradation of cytoplasmic components. The attachment of Apg12p, a modifier with no significant similarity to ubiquitin, to Apg5p is crucial for autophagy in yeast. This reaction proceeds in a ubiquitination-like manner, and requires Apg7p and Apg10p. Apg7p exhibits a considerable similarity to ubiquitin-activating enzyme (E1) and is found to activate Apg12p with ATP hydrolysis. Apg10p, on the other hand, shows no significant similarity to other proteins whose functions are known. Here, we show that after activation by Apg7p, Apg12p is transferred to the Cys-133 residue of Apg10p to form an Apg12p-Apg10p thioester. Cells expressing Apg10p(C133S) do not generate the Apg12p-Apg5p conjugate, which leads to defects in autophagy and cytoplasm-to-vacuole targeting of aminopeptidase I. These findings indicate that Apg10p is a new type of protein-conjugating enzyme that functions in the Apg12p-Apg5p conjugation pathway.

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  • Widely-used LC3 antibodies, such as the LC3B antibody from Cell Signaling Technology [2775] that targets the N terminus of LC3B, cannot recognize the amino terminal HiBiT-tagged LC3 resolved on protein blots.

    “One caveat to the immunostaining validation of cells that stably express LC3-HiBiT is the inability of widely-used LC3 antibodies (such as the LC3B antibody from Cell Signaling Technology [2775] that targets the N terminus of LC3B) to recognize the amino terminal HiBiT-tagged LC3 resolved on protein blots.”

    From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [2775]

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Metadata

Authors
Shintani, T., Mizushima, N., Ogawa, Y., Matsuura, A., Noda, T., & Ohsumi, Y.
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
1999-10-01
Volume
18
Issue
19
Pages
5234-5241
ISSN
  • 0261-4189
  • 1460-2075
DOI
10.1093/emboj/18.19.5234
Cited by
269
References
0
Language
en
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