Direct imaging of lateral movements of AMPA receptors inside synapses
Open accessAbstract
Trafficking of AMPA receptors in and out of synapses is crucial for synaptic plasticity. Previous studies have focused on the role of endo/exocytosis processes or that of lateral diffusion of extra-synaptic receptors. We have now directly imaged AMPAR movements inside and outside synapses of live neurons using single-molecule fluorescence microscopy. Inside individual synapses, we found immobile and mobile receptors, which display restricted diffusion. Extra-synaptic receptors display free diffusion. Receptors could also exchange between these membrane compartments through lateral diffusion. Glutamate application increased both receptor mobility inside synapses and the fraction of mobile receptors present in a juxtasynaptic region. Block of inhibitory transmission to favor excitatory synaptic activity induced a transient increase in the fraction of mobile receptors and a decrease in the proportion of juxtasynaptic receptors. Altogether, our data show that rapid exchange of receptors between a synaptic and extra-synaptic localization occurs through regulation of receptor diffusion inside synapses.
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Glutamate stimulation enhances exchange between synaptic and extrasynaptic receptor pools and increases overall motility of glutamate receptors.
“Glutamate stimulation enhances the exchange between these pools and increases overall motility of glutamate receptors (Tardin et al., 2003).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Tardin et al., 2003
The Regulation of AMPARs mobility inside synapses section reports that bath-applied glutamate increased diffusion within synapses and reduced the proportion of immobile synaptic receptors. The Discussion links these changes to movement from synapses into extrasynaptic membrane, supporting enhanced exchange. But the same results section says extrasynaptic receptor diffusion was not significantly changed, so the claim of increased overall motility is broader than the finding. (LLM, conf=0.86)
Experimental — accuracy not yet measuredA pool of synaptic receptors is immobile while another synaptic pool and extrasynaptic receptors are rapidly moving.
“A pool of synaptic receptors was shown to be immobile while another synaptic pool and extrasynaptic receptors were rapidly moving.”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Tardin et al., 2003
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References 48 · Cited by 384
- Authors
- Tardin, C., Cognet, L., Bats, C., Lounis, B., & Choquet, D.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Choquet, Daniel
- Venue
- The EMBO Journal
- Published
- 2003-09-15
- Volume
- 22
- Issue
- 18
- Pages
- 4656-4665
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC212729
- OpenAlex
- W2084807003
- Cited by
- 384
- References
- 48
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 8 · 2023: 8 · 2022: 7 · 2021: 14 · 2020: 9 · 2019: 9 · 2018: 17 · 2017: 11 · 2016: 12 · 2015: 15 · 2014: 24 · 2013: 19 · 2012: 29
- FWCI
- 6.83
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- arXiv (Cornell University) (submittedVersion)
- HAL (submittedVersion, other-oa)
- UCL Discovery (University College London) (submittedVersion)
- Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
- VU Research Portal (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- arXiv (Cornell University)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- AMPA receptors, receptor trafficking, synaptic plasticity, lateral diffusion, receptor mobility, glutamate, synaptic inhibition, single-molecule fluorescence microscopy, live neurons
- MeSH terms
- Animals, Bicuculline, Cells, Cultured, Diffusion, Embryo, Mammalian, Hippocampus, Microscopy, Fluorescence, Monosaccharide Transport Proteins, Neuronal Plasticity, Neurons, Synapses, Tetrodotoxin, Glutamic Acid, Glucose Transporter Type 2, Rats
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