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Biofilm formation-defective mutants in Pseudomonas putida

López-Sánchez, Aroa, Leal-Morales, Antonio, Jiménez-Díaz, Lorena, Platero, Ana I., Bardallo-Pérez, Juan, Díaz-Romero, Alberto, Acemel, Rafael D., Illán, Juan M., Jiménez-López, Julia, Govantes, Fernando·2016·FEMS Microbiology Letters
DOI 10.1093/femsle/fnw127
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Abstract

Out of 8000 candidates from a genetic screening for Pseudomonas putida KT2442 mutants showing defects in biofilm formation, 40 independent mutants with diminished levels of biofilm were analyzed. Most of these mutants carried insertions in genes of the lap cluster, whose products are responsible for synthesis, export and degradation of the adhesin LapA. All mutants in this class were strongly defective in biofilm formation. Mutants in the flagellar regulatory genes fleQ and flhF showed similar defects to that of the lap mutants. On the contrary, transposon insertions in the flagellar structural genes fliP and flgG, that also impair flagellar motility, had a modest defect in biofilm formation. A mutation in gacS, encoding the sensor element of the GacS/GacA two-component system, also had a moderate effect on biofilm formation. Additional insertions targeted genes involved in cell envelope function: PP3222, encoding the permease element of an ABC-type transporter and tolB, encoding the periplasmic component of the Tol-OprL system required for outer membrane stability. Our results underscore the central role of LapA, suggest cross-regulation between motility and adhesion functions and provide insights on the role of cell envelope trafficking and maintenance for biofilm development in P. putida.

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  • LapA is a massive surface adhesion protein (the largest polypeptide in strain KT2440) that participates in assembling cells during biofilm formation.

    “LapA is a massive surface adhesion protein (the largest polypeptide in strain KT2440) that participates in assembling cells during biofilm formation (Benedetti et al., 2016; López‐Sánchez et al., 2016).”

    From Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride (2022) · cites this paper as López‐Sánchez et al., 2016

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Research works printed but not linked: 64

  1. 10.1128/AEM.01233-10 · DOI
  2. Arora, 1997, A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner, J Bacteriol, 179, 5574, 10.1128/jb.179.17.5574-5581.1997 · DOI
  3. 10.1111/j.1365-2958.2009.06991.x · DOI
  4. 10.1128/JB.183.23.6787-6793.2001 · DOI
  5. 10.1016/j.mimet.2005.06.001 · DOI
  6. 10.1146/annurev.mi.49.100195.003431 · DOI
  7. 10.1126/science.284.5418.1318 · DOI · Paper page
  8. 10.1046/j.1365-2958.2003.03740.x · DOI
  9. 10.1128/MMBR.64.4.847-867.2000 · DOI
  10. 10.1146/annurev.biochem.73.011303.073626 · DOI
  11. de Lorenzo, 1990, Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria, J Bacteriol, 172, 6568, 10.1128/jb.172.11.6568-6572.1990 · DOI
  12. DeFlaun, 1990, Development of an adhesion assay and characterization of an adhesion-deficient mutant of Pseudomonas fluorescens, Appl Environ Microb, 56, 112, 10.1128/AEM.56.1.112-119.1990 · DOI
  13. 10.1111/j.1462-2920.2012.02732.x · DOI
  14. 10.1128/JB.182.9.2363-2369.2000 · DOI
  15. 10.1073/pnas.76.4.1648 · DOI
  16. 10.1073/pnas.78.12.7458 · DOI
  17. 10.1111/j.1365-2958.2009.06793.x · DOI
  18. 10.1111/j.1462-2920.2005.00775.x · DOI
  19. 10.1111/j.1574-6968.2006.00493.x · DOI
  20. 10.1016/S0022-2836(83)80284-8 · DOI
  21. Heeb, 2001, Regulatory roles of the GacS/GacA two-component system in plant-associated and other Gram-negative bacteria, Mol Plant Microbe In, 14, 1351, 10.1094/MPMI.2001.14.12.1351 · DOI
  22. 10.1111/j.1365-2958.2008.06281.x · DOI
  23. 10.1046/j.1365-2958.2003.03615.x · DOI
  24. 10.1099/mic.0.28696-0 · DOI
  25. 10.1016/S0022-2836(05)80266-9 · DOI
  26. Jiménez-Fernández, 2015, The c-di-GMP phosphodiesterase BifA regulates biofilm development in Pseudomonas putida, Environ Microbiol Rep, 7, 78, 10.1111/1758-2229.12153 · DOI
  27. 10.1111/mmi.12221 · DOI
  28. Kinscherf, 1999, Swarming by Pseudomonas syringae B728a Requires gacS (lemA) and gacA but not the acyl-homoserine lactone biosynthetic gene ahlI, J Bacteriol, 181, 4133, 10.1128/JB.181.13.4133-4136.1999 · DOI
  29. 10.1111/j.1574-6968.2006.00280.x · DOI
  30. 10.1093/jb/mvj010 · DOI
  31. 10.1111/j.1365-2958.2007.06042.x · DOI
  32. 10.1016/j.resmic.2005.11.003 · DOI
  33. 10.1128/JB.182.17.4764-4772.2000 · DOI
  34. 10.1128/JB.185.1.184-195.2003 · DOI
  35. López-Sánchez, 2013, New methods for the isolation and characterization of biofilm-persistent mutants in Pseudomonas putida, Environ Microbiol Rep, 5, 679, 10.1111/1758-2229.12067 · DOI
  36. Mao, 2009, DOOR: a Database of prOkaryotic OpeRons, Nucleic Acids Res, 37, 459, 10.1093/nar/gkn757 · DOI
  37. Martin dos Santos, 2004, Genomic features of Pseudomonas putida strain KT2440, Vol. 1, 79, 10.1007/978-1-4419-9086-0_3 · DOI
  38. Martínez-Gil, 2014, Roles of cyclic di-GMP and the Gac system in transcriptional control of the genes coding for the Pseudomonas putida adhesins LapA and LapF, J Bacteriol, 96, 1484, 10.1128/JB.01287-13 · DOI
  39. 10.1111/j.1365-2958.2010.07249.x · DOI
  40. 10.1371/journal.pone.0031765 · DOI
  41. 10.1111/j.1462-2920.2007.01286.x · DOI
  42. 10.1111/j.1462-2920.2011.02499.x · DOI
  43. 10.1016/j.tim.2005.05.009 · DOI
  44. Minamino, 1999, Components of the Salmonella flagellar export apparatus and classification of export substrates, J Bacteriol, 181, 1388, 10.1128/JB.181.5.1388-1394.1999 · DOI
  45. 10.1016/j.tim.2008.11.001 · DOI
  46. 10.1128/JB.00790-06 · DOI
  47. 10.1128/JB.05483-11 · DOI
  48. 10.1111/j.1462-2920.2011.02447.x · DOI
  49. 10.1146/annurev.micro.54.1.49 · DOI · Paper page
  50. 10.1046/j.1365-2958.1998.01062.x · DOI · Paper page
  51. 10.1006/meth.1999.0762 · DOI
  52. 10.1046/j.1365-2958.2000.01859.x · DOI
  53. Parkinson, 1976, cheA, cheB, and cheC genes of Escherichia coli and their role in chemotaxis, J Bacteriol, 126, 758, 10.1128/JB.126.2.758-770.1976 · DOI
  54. Rodríguez-Herva, 2010, Physiological and transcriptomic characterization of a fliA mutant of Pseudomonas putida KT2440, Environ Microbiol Rep, 2, 373, 10.1111/j.1758-2229.2009.00084.x · DOI
  55. 10.1128/AEM.00414-12 · DOI
  56. 10.1128/AEM.71.9.5404-5410.2005 · DOI
  57. 10.1111/j.1462-2920.2005.00797.x · DOI
  58. 10.1016/j.resmic.2007.04.001 · DOI
  59. 10.1111/j.1574-6941.2001.tb00788.x · DOI
  60. 10.1111/j.1365-2958.2004.04008.x · DOI
  61. 10.1111/j.1365-2958.2005.04648.x · DOI
  62. 10.1128/AEM.67.3.1400-1403.2001 · DOI
  63. Yang, 1994, Mutations affecting hyphal colonization and pyoverdine production in pseudomonads antagonistic toward Phytophthora parasitica, Appl Environ Microb, 60, 473, 10.1128/AEM.60.2.473-481.1994 · DOI
  64. 10.1111/j.1574-6968.2008.01339.x · DOI

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Metadata

Authors
López-Sánchez, A., Leal-Morales, A., Jiménez-Díaz, L., Platero, A. I., Bardallo-Pérez, J., Díaz-Romero, A., Acemel, R. D., Illán, J. M., Jiménez-López, J., & Govantes, F.
Venue
FEMS Microbiology Letters
Publisher
Oxford University Press (OUP)
Published
2016-05-11
Volume
363
Issue
13
Pages
fnw127
ISSN
1574-6968
DOI
10.1093/femsle/fnw127
Cited by
31
References
0
Language
en

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