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Streptococcal heme binding protein (Shp) promotes virulence and contributes to the pathogenesis of group A Streptococcus infection
Open accessAbstract
Streptococcal heme binding protein (Shp) is involved in the process of heme acquisition in group A Streptococcus (GAS). However, no research thus far has examined the contribution of Shp to the virulence of GAS. To this end, we generated an isogenic strain lacking the shp gene (Δshp) and its complemented strain (Δshp-c) using the parent strain MGAS5005 (WT). Deletion of shp increased survival rates and neutrophil recruitment and reduced skin lesion sizes and GAS loads in the blood and the liver, lung, kidney and spleen in subcutaneous infections of mice. These results indicate that Shp significantly contributes to the skin and systemic invasion of GAS. The growth of the Δshp mutant was significantly slower than MGAS5005 and Δshp-c than in non-immune human blood and in incubation with isolated rat neutrophils. Microarray transcriptional analyses found no alteration in expression of virulence genes, indicating that the phenotype of the Δshp mutant was directly linked to the lack of Shp. The findings indicate that Shp significantly contributes to GAS skin invasion, systemic infection and virulence and that these contributions of Shp are mediated by the effects of Shp on systemic GAS growth and neutrophil responses.
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“Our recent study (Zhang et al., 2017) found that Shp significantly contributes to GAS skin invasion, systemic infection, and virulence.”
From The Heme Transporter HtsABC of Group A Streptococcus Contributes to Virulence and Innate Immune Evasion in Murine Skin Infections (2018) · cites this paper as Zhang et al., 2017
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“Our laboratory recently reported that another heme uptake protein, Shp, is important for GAS virulence (Zhang et al., 2017).”
From The Heme Transporter HtsABC of Group A Streptococcus Contributes to Virulence and Innate Immune Evasion in Murine Skin Infections (2018) · cites this paper as Zhang et al., 2017
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Cites
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Song, Y., Zhang, X., Cai, M., Lv, C., Zhao, Y., Wei, D., & Zhu, H. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01105 · Cited by 14
“Our recent study (Zhang et al., 2017) found that Shp significantly contributes to GAS skin invasion, systemic infection, and virulence.”
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Metadata
- Authors
- Zhang, X., Lu, C., Zhang, F., Song, Y., Cai, M., & Zhu, H.
- Affiliations
- Harbin Medical University (CN)
- Corresponding author
- Zhang, Xiaolan
- Lu, Chunmei
- Zhu, Hui
- Venue
- Pathogens and Disease
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-07-21
- Volume
- 75
- Issue
- 7
- ISSN
- 2049-632X (ISSN-L)
- OpenAlex
- W2737343939
- Cited by
- 8
- References
- 24
- Citations by year
- 2024: 2 · 2020: 4 · 2018: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Pathogens and Disease (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese National Natural Science Foundation (81571957)
- Youth research and innovation foundation of Harbin Medical University (2017JCZX44, 2016JCZX35)
- Bureau of Harbin city science and technology fundation (2013RFLXJ021)
- China Postdoctoral Science Foundation (2014M551267)
- Heilongjiang Provincial Postdoctoral Science Foundation (LBH-Z13142)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Streptococcal Infections and Treatments
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and Maternal Infections
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- SHP, Streptococcus pyogenes, virulence factors, heme acquisition, neutrophil recruitment, dermal invasion, systemic infection, subcutaneous infection, mouse model
- MeSH terms
- Heme-Binding Proteins, Animals, Bacterial Proteins, Carrier Proteins, Genetic Complementation Test, Hemeproteins, Kidney, Liver, Lung, Neutrophils, Skin, Spleen, Streptococcal Infections, Streptococcus pyogenes, Virulence, Gene Expression, Gene Expression Regulation, Bacterial, Survival Analysis, Gene Deletion, Neutrophil Infiltration, Microarray Analysis, Mice
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- Zhang, X., Lu, C., Zhang, F., Song, Y., Cai, M., & Zhu, H. (2017). Streptococcal heme binding protein (Shp) promotes virulence and contributes to the pathogenesis of group A Streptococcus infection. Pathogens and Disease. https://doi.org/10.1093/femspd/ftx085
