The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
A Genetic Method for Generating Drosophila Eyes Composed Exclusively of Mitotic Clones of a Single Genotype
Abstract
The genetic analysis of a gene at a late developmental stage can be impeded if the gene is required at an earlier developmental stage. The construction of mosaic animals, particularly in Drosophila, has been a means to overcome this obstacle. However, the phenotypic analysis of mitotic clones is often complicated because standard methods for generating mitotic clones render mosaic tissues that are a composite of both mutant and phenotypically normal cells. We describe here a genetic method (called EGUF/hid) that uses both the GAL4/UAS and FLP/FRT systems to overcome this limitation for the Drosophila eye by producing genetically mosaic flies that are otherwise heterozygous but in which the eye is composed exclusively of cells homozygous for one of the five major chromosome arms. These eyes are nearly wild type in size, morphology, and physiology. Applications of this genetic method include phenotypic analysis of existing mutations and F1 genetic screens to identify as yet unknown genes involved in the biology of the fly eye. We illustrate the utility of the method by applying it to lethal mutations in the synaptic transmission genes synaptotagmin and syntaxin.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
Brand, A. H., & Perrimon, N. (1993) · Development · doi:10.1242/dev.118.2.401 · Cited by 9,612
The development of Drosophila melanogaster
Ingham, P. (1994) · Trends in Genetics · doi:10.1016/0168-9525(90)90017-z · Cited by 3,149
The Genome of Drosophila Melanogaster
Lindsley, D. L., & Zimm, G. G. (1992) · Cited by 3,098
Analysis of genetic mosaics in developing and adult Drosophila tissues
Xu, T., & Rubin, G. M. (1993) · Development · doi:10.1242/dev.117.4.1223 · Cited by 2,041
Homology of the eyeless Gene of Drosophila to the Small eye Gene in Mice and Aniridia in Humans
Quiring, R., Walldorf, U., Kloter, U., & Gehring, W. J. (1994) · Science · doi:10.1126/science.7914031 · Cited by 1,100
Expression of baculovirus P35 prevents cell death in Drosophila
Hay, B. A., Wolff, T., & Rubin, G. M. (1994) · Development · doi:10.1242/dev.120.8.2121 · Cited by 1,066
The FLP recombinase of yeast catalyzes site-specific recombination in the drosophila genome
Golic, K. G., & Lindquist, S. (1989) · Cell · doi:10.1016/0092-8674(89)90033-0 · Cited by 942
Simon, M. A., Bowtell, D. D. L., Dodson, G. S., Laverty, T. R., & Rubin, G. M. (1991) · Cell · doi:10.1016/0092-8674(91)90065-7 · Cited by 859
The head involution defective gene of Drosophila melanogaster functions in programmed cell death.
Grether, M. E., Abrams, J. M., Agapite, J., White, K., & Steller, H. (1995) · Genes & Development · doi:10.1101/gad.9.14.1694 · Cited by 699
BEHAVIORAL MUTANTS OF Drosophila ISOLATED BY COUNTERCURRENT DISTRIBUTION
Benzer, S. (1967) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.58.3.1112 · Cited by 672
Cited by
Glial Lipid Droplets and ROS Induced by Mitochondrial Defects Promote Neurodegeneration
Liu, L., Zhang, K., Sandoval, H., Yamamoto, S., Jaiswal, M., Sanz, E., Li, Z., Hui, J., Graham, B. H., Quintana, A., et al. (2015) · Cell · doi:10.1016/j.cell.2014.12.019 · Cited by 876
Verstreken, P., Ly, C. V., Venken, K. J. T., Koh, T.-W., Zhou, Y., & Bellen, H. J. (2005) · Neuron · doi:10.1016/j.neuron.2005.06.018 · Cited by 841
The art and design of genetic screens: Drosophila melanogaster
St Johnston, D. (2002) · Nature Reviews Genetics · doi:10.1038/nrg751 · Cited by 644
Igaki, T., Pastor-Pareja, J. C., Aonuma, H., Miura, M., & Xu, T. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.01.002 · Cited by 295
Murthy, M., Garza, D., Scheller, R. H., & Schwarz, T. L. (2003) · Neuron · doi:10.1016/s0896-6273(03)00031-x · Cited by 202
Self-citation
Metadata
- Authors
- Stowers, R. S., & Schwarz, T. L.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-08-01
- Volume
- 152
- Issue
- 4
- Pages
- 1631-1639
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2123859931
- Cited by
- 339
- References
- 30
- FWCI
- 3.78
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A Genetic Method for Generating Drosophila Eyes Composed Exclusively of Mitotic Clones of a Single Genotype. https://app.cochat.ai/papers/10.1093/genetics/152.4.1631
- The paper
- Stowers, R. S., & Schwarz, T. L. (1999). A Genetic Method for Generating Drosophila Eyes Composed Exclusively of Mitotic Clones of a Single Genotype. Genetics. https://doi.org/10.1093/genetics/152.4.1631
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
