The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. I. Genetic and Phenotypic Bases of Wrinkly Spreader Fitness
Abstract
A central feature of all adaptive radiations is morphological divergence, but the phenotypic innovations that are responsible are rarely known. When selected in a spatially structured environment, populations of the bacterium Pseudomonas fluorescens rapidly diverge. Among the divergent morphs is a mutant type termed “wrinkly spreader” (WS) that colonizes a new niche through the formation of self-supporting biofilms. Loci contributing to the primary phenotypic innovation were sought by screening a WS transposon library for niche-defective (WS-) mutants. Detailed analysis of one group of mutants revealed an operon of 10 genes encoding enzymes necessary to produce a cellulose-like polymer (CLP). WS genotypes overproduce CLP and overproduction of the polymer is necessary for the distinctive morphology of WS colonies; it is also required for biofilm formation and to maximize fitness in spatially structured microcosms, but overproduction of CLP alone is not sufficient to cause WS. A working model predicts that modification of cell cycle control of CLP production is an important determinant of the phenotypic innovation. Analysis of >30 kb of DNA encoding traits required for expression of the WS phenotype, including a regulatory locus, has not revealed the mutational causes, indicating a complex genotype-phenotype map.
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Metadata
- Authors
- Spiers, A. J., Kahn, S. G., Bohannon, J., Travisano, M., & Rainey, P. B.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-05-01
- Volume
- 161
- Issue
- 1
- Pages
- 33-46
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1909235440
- Cited by
- 281
- References
- 66
- FWCI
- 4.64
- Type
- journal article
- Language
- en
- Open access
- closed
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- Spiers, A. J., Kahn, S. G., Bohannon, J., Travisano, M., & Rainey, P. B. (2002). Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. I. Genetic and Phenotypic Bases of Wrinkly Spreader Fitness. Genetics. https://doi.org/10.1093/genetics/161.1.33
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