The DOI matches its Crossref record.
The Inheritance of Chemical Phenotype in Cannabis sativa L.
Abstract
Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Δ-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F1’s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual F1 plants were self-fertilized, and 10 inbred F2 offspring were collected and analyzed. In all cases, a segregation of the three chemotypes (pure CBD, mixed CBD-THC, and pure THC) fitting a 1:2:1 proportion was observed. The CBD/THC ratio was found to be significantly progeny specific and transmitted from each F1 to the F2’s derived from it. A model involving one locus, B, with two alleles, BD and BT, is proposed, with the two alleles being codominant. The mixed chemotypes are interpreted as due to the genotype BD/BT at the B locus, while the pure-chemotype plants are due to homozygosity at the B locus (either BD/BD or BT/BT). It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC, respectively. The F2 segregating groups were used in a bulk segregant analysis of the pooled DNAs for screening RAPD primers; three chemotype-associated markers are described, one of which has been transformed in a sequence-characterized amplified region (SCAR) marker and shows tight linkage to the chemotype and codominance.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects.
Russo, E. B. (2011) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2011.01238.x · Cited by 1,535
The draft genome and transcriptome of Cannabis sativa.
van Bakel, H., Stout, J. M., Cote, A. G., Tallon, C. M., Sharpe, A. G., Hughes, T. R., & Page, J. E. (2011) · Genome Biology · doi:10.1186/gb-2011-12-10-r102 · Cited by 575
Farinon, B., Molinari, R., Costantini, L., & Merendino, N. (2020) · Nutrients · doi:10.3390/nu12071935 · Cited by 473
Evolution and classification of Cannabis sativa (marijuana, hemp) in relation to human utilization.
Small, E. (2015) · The Botanical Review · doi:10.1007/s12229-015-9157-3 · Cited by 461
Phytocannabinoids as novel therapeutic agents in {CNS} disorders.
Hill, A. J., Williams, C. M., Whalley, B. J., & Stephens, G. J. (2012) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.09.002 · Cited by 312
Phytochemical and genetic analyses of ancient Cannabis from Central Asia.
Russo, E. B., Jiang, H.-E., Li, X., Sutton, A., Carboni, A., del Bianco, F., Mandolino, G., Potter, D. J., Zhao, Y.-X., Bera, S., et al. (2008) · Journal of Experimental Botany · doi:10.1093/jxb/ern260 · Cited by 261
Self-citationThe effects of cannabinoids on P-glycoprotein transport and expression in multidrug resistant cells
Holland, M. L., Panetta, J. A., Hoskins, J. M., Bebawy, M., Roufogalis, B. D., Allen, J. D., & Arnold, J. C. (2006) · Biochemical Pharmacology · doi:10.1016/j.bcp.2005.12.033 · Cited by 157
Pacifico, D., Miselli, F., Carboni, A., Moschella, A., & Mandolino, G. (2008) · Euphytica · doi:10.1007/s10681-007-9543-y · Cited by 147
Self-citationIdentification of candidate genes affecting Δ9-tetrahydrocannabinol biosynthesis in Cannabis sativa
Marks, M. D., Tian, L., Wenger, J. P., Omburo, S. N., Soto-Fuentes, W., He, J., Gang, D. R., Weiblen, G. D., & Dixon, R. A. (2009) · Journal of Experimental Botany · doi:10.1093/jxb/erp210 · Cited by 125
Cannabinoids and terpenes as chemotaxonomic markers in cannabis.
Fischedick J, E. S. (2015) · Natural Products Chemistry & Research · doi:10.4172/2329-6836.1000181 · Cited by 109
Phytocannabinoids in Cannabis sativa: Recent Studies on Biosynthetic Enzymes
Taura, F., Sirikantaramas, S., Shoyama, Y., Shoyama, Y., & Morimoto, S. (2007) · Chemistry & Biodiversity · doi:10.1002/cbdv.200790145 · Cited by 98
Cannabinoids: new promising agents in the treatment of neurological diseases.
Giacoppo, S., Mandolino, G., Galuppo, M., Bramanti, P., & Mazzon, E. (2014) · Molecules · doi:10.3390/molecules191118781 · Cited by 79
Self-citationIoannidis, K., Tomprou, I., Mitsis, V., & Koropouli, P. (2022) · Plants · doi:10.3390/plants11192569 · Cited by 31
Metadata
- Authors
- de Meijer, E. P. M., Bagatta, M., Carboni, A., Crucitti, P., Moliterni, V. M. C., Ranalli, P., & Mandolino, G.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-01-01
- Volume
- 163
- Issue
- 1
- Pages
- 335-346
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2169449092
- Cited by
- 354
- References
- 0
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
