Heteroduplex repair as an intermediate step of UV mutagenesis in yeast
Abstract
We have measured UV-induced mutation frequencies in yeast in a forward, nonselective assay system by scoring white adex ade2 double auxotrophs among parental red-pigmented ade2 clones. The frequencies of sectored and pure mutant clones were determined separately. In excision-defective strains carrying the genes radl-1, rad3-2 and rad4-4, as well as in the double mutants, rad 1-1 rad 3-2 and rad 1-1 rad 4-4, considerably more sectored than pure clones are induced in the low-dose range; in repair-competent strains, pure mutant clones substantially outnumber the sectored clones. These results can be explained on the basis of known differences in the timing of error-prone repair during the cell division cycle; that is, we assume that error-prone repair occurs primarily before replication in RAD wild-type strains but after replication in excision-deficient mutants. It has been suggested that excision deficiency has a pleiotropic effect on heteroduplex repair and nucleotide excision repair; however, the high percentage (36.6%) of half-sectored clones found in the rad1-1 strain is hard to reconcile with this hypothesis. We propose that heteroduplex repair occurs subsequent to error-prone repair in both excisionproficient and excision-deficient strains.
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Closely opposing lesions may contribute to explaining that in wild-type yeast and bacteria most UV-induced mutagenesis depends on functional NER and occurs in G1, while without NER mutagenesis is S-phase specific.
“These particular lesions may also contribute to explain the observation that both in wild-type yeast and bacteria cells most of the UV-induced mutagenesis depends upon a functional NER and takes place in G1 cells, while in the absence of NER the mutagenesis is S-phase specific [80,81].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [80]
Cites
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Self-citationUV-induced lethal sectoring and pure mutant clones in yeast
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Giannattasio, M., Follonier, C., Tourrière, H., Puddu, F., Lazzaro, F., Pasero, P., Lopes, M., Plevani, P., & Muzi-Falconi, M. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.09.004 · Cited by 121 · see citing works
Mind the gap: keeping UV lesions in check
Novarina, D., Amara, F., Lazzaro, F., Plevani, P., & Muzi-Falconi, M. (2011) · DNA Repair · doi:10.1016/j.dnarep.2011.04.030 · Cited by 43 · see citing works
- Authors
- Eckardt, F., Teh, S.-J., & Haynes, R. H.
- Affiliations
- York University (CA)
- Corresponding author
- Eckardt, Friederike
- Teh, Soo-Jeet
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1980-05-01
- Volume
- 95
- Issue
- 1
- Pages
- 63-80
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1944821569
- Cited by
- 28
- References
- 6
- Citations by year
- 2021: 1 · 2014: 1 · 2013: 1
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- UV mutagenesis, error-prone repair, nucleotide excision repair, RAD1, Rad3, Rad4, Saccharomyces cerevisiae
- MeSH terms
- Clone Cells, DNA Repair, DNA, Fungal, Mutation, Pyrimidine Dimers, Saccharomyces cerevisiae, Ultraviolet Rays
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