The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Cytokines in the follicular fluid of stimulated and non-stimulated human ovaries; is ovulation a suppressed inflammatory reaction?
Abstract
We determined the concentrations of tumour necrosis factor (TNF)-alpha, interleukins (IL)-1 beta, -6, -8 and -1-receptor antagonist (IL-1-ra) and of oestradiol and progesterone in the follicular fluid of 111 women undergoing in-vitro fertilization (IVF) and of six women with ovarian cysts in order to elucidate mid-cycle mechanisms causing dissociation of the follicle wall and local rupture of the ovarian tissue complex. Four stimulation protocols were administered: gonadotrophin releasing hormone agonist/human menopausal gonadotrophin (GnRHa/HMG), clomiphene citrate/HMG (CC/HMG), HMG and follicle-stimulating hormone (FSH). Concentrations of TNF alpha and IL-1 beta were below 15 and 3 pg/ml respectively. IL-6 (median 4.1, 3.5-4.4 pg/ml, 95% CI) was higher after stimulation with FSH (5.6 pg/ml) than with HMG (3.2 pg/ml, P < 0.05) or GnRHa/HMG (3.7 pg/ml, P < 0.05), and after stimulation with CC/HMG (5.5 pg/ml) than with HMG (P < 0.01) or GnRHa/HMG (P < 0.001). IL-8 ranged from 32 to 1241 pg/ml (147, 117-178 pg/ml) and IL-1-ra from < 31 to > 10,000 pg/ml (156, 109-192 pg/ml). Cytokine levels did not correlate to oestradiol or progesterone concentrations. The ovarian cysts contained similar IL-8 (14-540 pg/ml) and IL-1 beta (< 30 pg/ml), but higher IL-6 (13.6-> 500 pg/ml) and lower IL-1-ra concentrations. We assume that IL-6, IL-8 and IL-1-ra are involved in peri-ovulatory cellular interactions. Thus, ovulation appears to be a cytokine-regulated process of an 'inflammation' (IL-6 and IL-8) followed by 'anti-inflammatory' reactions (IL-1-ra).
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“Ovulation is a complex event regulated by the hypothalamicpituitary ovarian axis and also involves local chemokines and other factors related to inflammation”
From Chronic pelvic pain and endometriosis: translational evidence of the relationship and implications (2011) · cites this paper as Buscher et al., 1999
Citations
Cites
Blocking IL-1: interleukin 1 receptor antagonist in vivo and in vitro
Dinarello, C. A., & Thompson, R. C. (1991) · Immunology Today · doi:10.1016/0167-5699(91)90142-g · Cited by 724
REGULATION OF OVARIAN FOLLICLE ATRESIA
Kaipia, A., & Hsueh, A. J. W. (1997) · Annual Review of Physiology · doi:10.1146/annurev.physiol.59.1.349 · Cited by 455
Ovarian steroid treatment blocks a postmenopausal increase in blood monocyte interleukin 1 release.
Pacifici, R., Rifas, L., McCracken, R., Vered, I., McMurtry, C., Avioli, L. V., & Peck, W. A. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.7.2398 · Cited by 375
Pottratz, S. T., Bellido, T., Mocharla, H., Crabb, D., & Manolagas, S. C. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117100 · Cited by 274
Effect of estradiol on interleukin 1 synthesis by macrophages
Hu, S. K., Mitcho, Y. L., & Rath, N. C. (1988) · International Journal of Immunopharmacology · doi:10.1016/0192-0561(88)90055-0 · Cited by 171
Bonello, N., McKie, K., Jasper, M. J., Andrew, L., Ross, N., Braybon, E., Brännström, M., & Norman, R. J. (1996) · Biology of Reproduction · doi:10.1095/biolreprod54.2.436 · Cited by 166
Hurwitz, A., Loukides, J. A., Ricciarelli, E., Botero, L., Katz, E., McAllister, J. M., Garcia, J. E., Rohan, R. M., Adashi, E. Y., & Hernández, E. R. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115777 · Cited by 154
Granulosa cells as a source and target organ for tumor necrosis factor‐α
Zolti, M., Meirom, R., Shemesh, M., Wollach, D., Mashiach, S., Shore, L. S., & Ben‐Rafael, Z. (1990) · FEBS Letters · doi:10.1016/0014-5793(90)80565-z · Cited by 140
Wang, L. J., Brännström, M., Robertson, S. A., & Norman, R. J. (1992) · Fertility and Sterility · doi:10.1016/s0015-0282(16)55438-7 · Cited by 130
Human Follicular Fluids Contain Tissue Macrophages*
Loukides, J. A., Loy, R. A., EDWARDS, R., Honig, J., Visintin, I., & Polan, M. L. (1990) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-71-5-1363 · Cited by 127
Cited by
Ovulation: Parallels With Inflammatory Processes
Duffy, D. M., Ko, C., Jo, M., Brannstrom, M., & Curry, T. E. (2019) · Endocrine Reviews · doi:10.1210/er.2018-00075 · Cited by 513
Chronic pelvic pain and endometriosis: translational evidence of the relationship and implications
Stratton, P., & Berkley, K. J. (2011) · Human Reproduction Update · doi:10.1093/humupd/dmq050 · Cited by 442
New Insights Into the Pathogenesis of Serous Ovarian Cancer and Its Clinical Impact
Levanon, K., Crum, C., & Drapkin, R. (2008) · Journal of Clinical Oncology · doi:10.1200/jco.2008.18.1107 · Cited by 412
Vanderzwalmen, P., Bertin, G., Debauche, C., Standaert, V., van Roosendaal, E., Vandervorst, M., Bollen, N., Zech, H., Mukaida, T., Takahashi, K., et al. (2002) · Human Reproduction · doi:10.1093/humrep/17.3.744 · Cited by 280
Inflammation and Human Ovarian Follicular Dynamics
Boots, C. E., & Jungheim, E. S. (2015) · Seminars in Reproductive Medicine · doi:10.1055/s-0035-1554928 · Cited by 255
Metadata
- Authors
- Buscher, U.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-01-01
- Volume
- 14
- Issue
- 1
- Pages
- 162-166
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2013876693
- Cited by
- 109
- References
- 28
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 4 · 2018: 6 · 2017: 6 · 2016: 5 · 2015: 1 · 2014: 7 · 2013: 5 · 2012: 2
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Reproductive Physiology in Livestock
- Keywords
- follicular fluid, cytokines, ovulation, interleukin-6, interleukin-8, interleukin-1 receptor antagonist, TNF-α, IL-1β, ovulation induction, follicle rupture, ovarian cysts, estradiol, progesterone, in vitro fertilization, inflammation, gonadotropins
- MeSH terms
- Adult, Cell Count, Female, Ovarian Follicle, Humans, Middle Aged, Oocytes, Ovarian Cysts, Ovary, Ovulation, Specimen Handling, Stimulation, Chemical, Tumor Necrosis Factor-alpha, Follicular Fluid, Interleukin-6, Cytokines, Interleukin-8, Receptors, Interleukin-1
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