The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development
Abstract
BACKGROUND: The integrity of sperm DNA is important for the success of natural or assisted fertilization, as well as normal development of the embryo, fetus and child. ICSI, by bypassing sperm selection mechanisms, increases the risk of transmitting damaged DNA and the significance of this requires investigation. METHODS: DNA damage in sperm from an unselected group of 60 men undergoing IVF treatment was measured by single cell gel electrophoresis (Comet assay) and correlated with semen and treatment cycle parameters. RESULTS: Wide spectra of sperm DNA damage were found both within and between men but no specific subgroups were identified. Semen and treatment cycle parameters were not different in men grouped according to high or low sperm DNA damage. However, regression analysis showed that DNA damage was positively associated with age (29-44 years), abnormal sperm and motility and negatively associated with sperm concentration. In ICSI cycles DNA damage was positively associated with impairment of post-fertilization embryo cleavage. CONCLUSIONS: This study contributes to the evidence of DNA damage within sperm. High loads of DNA damage measured by the Comet assay were predictive of failure of embryo development after ICSI. As it is likely that sperm with DNA damage contributed to successful fertilization and in-vitro development, potential adverse effects remain to be clarified.
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Citations
Cites
Donnelly, E. T. (2001) · Human Reproduction · doi:10.1093/humrep/16.6.1191 · Cited by 263
Cited by
The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis
Robinson, L., Gallos, I. D., Conner, S. J., Rajkhowa, M., Miller, D., Lewis, S., Kirkman-Brown, J., & Coomarasamy, A. (2012) · Human Reproduction · doi:10.1093/humrep/des261 · Cited by 629
Function of sperm chromatin structural elements in fertilization and development
Ward, W. S. (2010) · Molecular Human Reproduction · doi:10.1093/molehr/gap080 · Cited by 395
Zhao, J., Zhang, Q., Wang, Y., & Li, Y. (2014) · Fertility and Sterility · doi:10.1016/j.fertnstert.2014.06.033 · Cited by 361
Sperm chromatin structure and male fertility: biological and clinical aspects
Erenpreiss, J., Spano, M., Erenpreisa, J., Bungum, M., & Giwercman, A. (2006) · Asian Journal of Andrology · doi:10.1111/j.1745-7262.2006.00112.x · Cited by 304
Benchaib, M., Lornage, J., Mazoyer, C., Lejeune, H., Salle, B., & François Guerin, J. (2007) · Fertility and Sterility · doi:10.1016/j.fertnstert.2006.05.057 · Cited by 285
Metadata
- Authors
- Morris, I. D.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-04-01
- Volume
- 17
- Issue
- 4
- Pages
- 990-998
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2112400063
- Cited by
- 520
- References
- 1
- FWCI
- 23.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- Morris, I. D. (2002). The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Human Reproduction. https://doi.org/10.1093/humrep/17.4.990
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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