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Cited by 41Antifungal resistance and susceptibilityOcular Infections and TreatmentsAntibiotics Pharmacokinetics and Efficacy

Antifungal activity of four tetracycline analogues against Candida albicans in vitro: potentiation by amphotericin B

Lew, M. A., Beckett, K. M., Levin, M. J.·1977·Journal of Infectious Diseases
DOI 10.1093/infdis/136.2.263
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Abstract

The antifungal activities of four tetracycline analogues in combination with amphotericin B (AmB) were determined against 20 strains of Candida albicans. When a microtiter checkerboard technique was used, minocycline (less than or equal to 10 microgram/ml) acted synergistically with AmB against all strains, whereas doxycycline had a reduced effect, and demeclocycline and tetracycline had no potentiating effect at this concentration. Killing-curve experiments with two strains of C. albicans demonstrated that the combination of minocycline and AmB produced a decrease in number of colony-forming units (cfu) of greater than 2 logs in 4 hr and a 4-log decrease in cfu in 24 hr at concentrations (minocycline, 0.64 microgram/ml; AmB, 0.1 microgram/ml) that were subinhibitory when each agent was used alone and that are readily achieved in human serum and body fluids with conventional doses. The killing-curve technique indicated that doxycycline had an intermediate degree of synergistic activity, whereas tetracycline had no synergistic activity at clinically relevant concentrations.

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Crossref · 10.1093/infdis/136.2.263

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  • Previous studies have suggested that AmB may facilitate the entry of tetracycline derivatives into the fungal cell.

    “Previous studies have suggested that AmB may facilitate the entry of tetracycline derivatives into the fungal cell [13]; however, the molecular mechanisms associated with AmB combined with NSAIDs against C. albicans planktonic cells or biofilms remain undefined.”

    From In vitro analysis of flufenamic acid activity against Candida albicans biofilms (2014) · cites this paper as [13]

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Authors
Lew, M. A., Beckett, K. M., & Levin, M. J.
Corresponding author
Lew, M. A.
ORCID
Levin, M. J.
Venue
Journal of Infectious Diseases
Publisher
Oxford University Press (OUP)
Published
1977-08-01
Volume
136
Issue
2
Pages
263-270
ISSN
  • 0022-1899 (ISSN-L)
  • 1537-6613
DOI
10.1093/infdis/136.2.263
OpenAlex
W2321486094
Cited by
41
References
36
Citations by year
2025: 2 · 2024: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
6.73
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Health Sciences › Medicine › Ophthalmology › Ocular Infections and Treatments
  • Health Sciences › Medicine › Pharmacology › Antibiotics Pharmacokinetics and Efficacy
Keywords
Candida albicans, tetracycline analogues, minocycline, doxycycline, demeclocycline, tetracycline, amphotericin B, antifungal synergy, combination antifungal therapy, killing curves, checkerboard assay, subinhibitory concentrations
MeSH terms
Amphotericin B, Candida albicans, Demeclocycline, Dose-Response Relationship, Drug, Doxycycline, Drug Synergism, Minocycline, Tetracycline, Tetracyclines

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