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HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in Western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy
Abstract
The mAb HCA2 reacts preferentially with HLA-A locus heavy chains. Its reactivity contrasts with that of HC10, a mAb with more pronounced specificity for HLA-B and -C heavy chains. Both HCA2 and HC10 were raised against free class I heavy chains of HLA-A and -B antigens respectively, to obtain mAbs that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HCA2 and HC10 indeed retain strong reactivity with free class I heavy chains in Western blots. The HCA2 mAb in particular reacts in a locus-specific manner by biochemical criteria. Conditions are described for use of HCA2 and HC10 in immunohistochemical staining of formalin-fixed, paraffin-embedded sections. HCA2 and HC10 also produce strong reactivity in immuno-electron microscopy. Their use allows the determination of tissue and subcellular distribution of class I antigens.
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Metadata
- Authors
- Stam, N. J., Vroom, T. M., Peters, P. J., Pastoors, E. B., & Ploegh, H. L.
- Venue
- International Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 2
- Issue
- 2
- Pages
- 113-125
- ISSN
- 0953-8178 (ISSN-L)
- 1460-2377
- OpenAlex
- W2076537666
- Cited by
- 250
- References
- 0
- FWCI
- 5.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in Western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. https://app.cochat.ai/papers/10.1093/intimm/2.2.113
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- Stam, N. J., Vroom, T. M., Peters, P. J., Pastoors, E. B., & Ploegh, H. L. (1990). HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in Western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. International Immunology. https://doi.org/10.1093/intimm/2.2.113
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