Chemical composition, toxicological aspects and antifungal activity of essential oil from Lippia sidoides Cham.

Fontenelle, R. O. S.; Morais, S. M.; Brito, E. H. S.; Kerntopf, M. R.; Brilhante, R. S. N.; Cordeiro, R. A.; Tomé, A. R.; Queiroz, M. G. R.; Nascimento, N. R. F.; Sidrim, J. J. C.; Rocha, M. F. G.

doi:10.1093/jac/dkm066

Cited by 181Essential Oils and Antimicrobial ActivityInsect Pest Control StrategiesPhytochemistry Medicinal Plant Applications

Chemical composition, toxicological aspects and antifungal activity of essential oil from Lippia sidoides Cham.

Fontenelle, R. O. S., Morais, S. M., Brito, E. H. S., Kerntopf, M. R., Brilhante, R. S. N., Cordeiro, R. A., Tomé, A. R., Queiroz, M. G. R. +3 more·2007·Journal of Antimicrobial Chemotherapy

DOI 10.1093/jac/dkm066

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Abstract

OBJECTIVES: The aims of this study were to test the essential oil from Lippia sidoides Cham. for antifungal activity, in vitro, against Candida spp. and Microsporum canis, to evaluate its acute and subchronic toxicological effects, in vivo, and to determine its chemical constituents. METHODS: The antifungal activity, in vitro, was initially evaluated by the agar-well diffusion technique, and the MIC and minimum fungicidal concentration (MFC) were determined by the broth microdilution method. The acute and subchronic toxicological effects were determined in mice and rats, respectively. The chemical composition of the essential oil was determined by gas chromatography coupled to mass spectroscopy. RESULTS: The essential oil obtained from L. sidoides was effective against all tested strains by the agar-well diffusion method. The MICs of L. sidoides essential oil for strains of M. canis ranged from 4 to 70 mg/L and the MFCs ranged from 9 to 150 mg/L. The MICs for strains of Candida spp. ranged from 620 to 2500 mg/L and the MFCs ranged from 1250 to 5000 mg/L. The main constituents of L. sidoides essential oil were thymol (59.65%), E-caryophyllene (10.60%) and p-cymene (9.08%). The acute administration of the essential oil up to 3 g/kg by the oral route to mice was devoid of overt toxicity. The 30 day oral administration of L. sidoides oil (117.95 mg/kg/day) to rats did not induce any significant histopathological, haematological or serum biochemical alterations. CONCLUSIONS: The essential oil from L. sidoides may be a promising source in the search for new antifungal drugs due to its efficacy and low toxicity.

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Authors: Fontenelle, R. O. S., Morais, S. M., Brito, E. H. S., Kerntopf, M. R., Brilhante, R. S. N., Cordeiro, R. A., Tomé, A. R., Queiroz, M. G. R., Nascimento, N. R. F., Sidrim, J. J. C., & Rocha, M. F. G.
Affiliations: Universidade Estadual do Ceará (BR)
Universidade Federal do Ceará (BR)
Corresponding author: Rocha, M. F. G.
ORCID: Morais, S. M.
Kerntopf, M. R.
Brilhante, R. S. N.
Queiroz, M. G. R.
Nascimento, N. R. F.
Sidrim, J. J. C.
Rocha, M. F. G.
Venue: Journal of Antimicrobial Chemotherapy
Publisher: Oxford University Press (OUP)
Published: 2007-03-21
Volume: 59
Issue: 5
Pages: 934-940
ISSN: 0305-7453 (ISSN-L)
1460-2091
DOI: 10.1093/jac/dkm066
OpenAlex: W2160213984
Cited by: 181
References: 35
Citations by year: 2026: 6 · 2025: 1 · 2024: 18 · 2023: 8 · 2022: 10 · 2021: 15 · 2020: 10 · 2019: 6 · 2018: 9 · 2017: 10 · 2016: 10 · 2015: 12 · 2014: 12 · 2013: 10 · 2012: 11
FWCI: 11.36
Type: journal article
Language: en
Open access: closed
Locations: Journal of Antimicrobial Chemotherapy (publishedVersion)
PubMed (publishedVersion)
HighWire Press Open Archive (submittedVersion)
Funders: Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry Medicinal Plant Applications
Keywords: Lippia sidoides, essential oils, antifungal activity, Candida, Microsporum canis, acute toxicity, subchronic toxicity, thymol, caryophyllene, p-cymene, chemical composition, minimum inhibitory concentration, minimum fungicidal concentration, antifungal drug development
MeSH terms: Animals, Antifungal Agents, Candida, Microsporum, Oils, Volatile, Rats, Wistar, Lippia, Mice, Rats

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Chemical composition, toxicological aspects and antifungal activity of essential oil from Lippia sidoides Cham.

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