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Use of Detergents in the Analysis of Fibrous Feeds. II. A Rapid Method for the Determination of Fiber and Lignin
Abstract
The capacity of cetyl trimethylammonium bromide to dissolve proteins in acid solution has been utilized in development of a method, called acid-detergent fiber method (ADF), which is not only a fiber determination in itself but also the major preparatory step in the determination of lignin. The entire procedure for determining fiber and lignin is considerably more rapid than presently published methods. Compositional studies show ADF to consist chiefly of lignin and polysaccharides. Correlations with the new fiber method and digestibility of 18 forages (r = —0.79) showed it to be somewhat superior to crude fiber (r = —0.73) in estimating nutritive value. The correlation of the new lignin method and digestibility was -0.90 when grass and legume species were separated.
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Citations
Cited by
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78551-2 · Cited by 28,658
Cotrufo, M. F., Wallenstein, M. D., Boot, C. M., Denef, K., & Paul, E. (2013) · Global Change Biology · doi:10.1111/gcb.12113 · Cited by 3,552
Nitrogen and Lignin Control of Hardwood Leaf Litter Decomposition Dynamics
Melillo, J. M., Aber, J. D., & Muratore, J. F. (1982) · Ecology · doi:10.2307/1936780 · Cited by 2,641
Carbohydrate and lignin contents of plant materials used in animal feeding
Knudsen, K. E. B. (1997) · Animal Feed Science and Technology · doi:10.1016/s0377-8401(97)00009-6 · Cited by 1,548
Bridgeman, T. G., Jones, J. M., Shield, I., & Williams, P. T. (2008) · Fuel · doi:10.1016/j.fuel.2007.05.041 · Cited by 915
Metadata
- Authors
- Soest, P. J. V.
- Venue
- Journal of AOAC INTERNATIONAL
- Publisher
- Oxford University Press (OUP)
- Published
- 1963-10-01
- Volume
- 46
- Issue
- 5
- Pages
- 829-835
- ISSN
- 0004-5756 (ISSN-L)
- 0095-9111
- OpenAlex
- W3006211509
- Cited by
- 2,566
- References
- 0
- FWCI
- 6.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Use of Detergents in the Analysis of Fibrous Feeds. II. A Rapid Method for the Determination of Fiber and Lignin. https://app.cochat.ai/papers/10.1093/jaoac/46.5.829
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- Soest, P. J. V. (1963). Use of Detergents in the Analysis of Fibrous Feeds. II. A Rapid Method for the Determination of Fiber and Lignin. Journal of AOAC INTERNATIONAL. https://doi.org/10.1093/jaoac/46.5.829
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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