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Histochemical Demonstration of Acid Phosphatases With Naphthol AS-Phosphates
Abstract
A simple, reliable, and convenient azo-dye method for the demonstration of acid phosphatase and phosphamidase in paraffin-embedded tissues is described. The best results are obtained with frozen-dried tissues. Phosphates of naphthols AS-BI, AS-BS, AS-LC, and AS-TR are useful substrates (text-fig. 1, II). The stability and potential high chromogenicity of these substrates suggest their use in quantitative determinations. The present study indicates that acid phosphatase is not as sensitive an enzyme as previously assumed, and that with reasonable care it can be preserved even in mounted sections over a long period.
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Cited by
Fuller, K., Wong, B., Fox, S., Choi, Y., & Chambers, T. J. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.5.997 · Cited by 561
Osteocytic osteolysis observed in rats to which parathyroid hormone was continuously administered
Tazawa, K., Hoshi, K., Kawamoto, S., Tanaka, M., Ejiri, S., & Ozawa, H. (2004) · Journal of Bone and Mineral Metabolism · doi:10.1007/s00774-004-0519-x · Cited by 124
Watanabe, Y., Namba, A., Aida, Y., Honda, K., Tanaka, H., Suzuki, N., Matsumura, H., & Maeno, M. (2009) · Mediators of Inflammation · doi:10.1155/2009/308596 · Cited by 13
SAEKI, M., TAKAHASHI-IWANAGA, H., IWANAGA, T., FUJITA, T., KODAMA, M., HANAWA, H., ZHANG, S., IZUMI, T., & SHIBATA, A. (1994) · The Tohoku Journal of Experimental Medicine · doi:10.1620/tjem.172.195 · Cited by 8
Metadata
- Venue
- JNCI: Journal of the National Cancer Institute
- Publisher
- Oxford University Press (OUP)
- Published
- 1958-09
- Volume
- 21
- Issue
- 3
- Pages
- 523-39
- ISSN
- 0027-8874 (ISSN-L)
- 1460-2105
- OpenAlex
- W2467596693
- Cited by
- 498
- References
- 0
- FWCI
- 10.13
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Histochemical Demonstration of Acid Phosphatases With Naphthol AS-Phosphates. https://app.cochat.ai/papers/10.1093/jnci/21.3.523
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- Histochemical Demonstration of Acid Phosphatases With Naphthol AS-Phosphates. (1958). JNCI: Journal of the National Cancer Institute. https://doi.org/10.1093/jnci/21.3.523
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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