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New Soluble-Formazan Assay for HIV-1 Cytopathic Effects: Application to High-Flux Screening of Synthetic and Natural Products for AIDS-Antiviral Activity
Abstract
We have developed an effective and optimally safe microculture method for rapid and convenient assay of the in vitro cytopathic effects of human immunodeficiency virus (HIV-1) on human lymphoblastoid or other suitable host cells. The assay procedure is applicable to the evaluation of drug effects on in vitro infections induced directly in cultured host cells by cell-free HIV-1 or by coculture with H9 cells chronically infected with HIV-1. The assay uses a newly developed tetrazolium reagent that is metabolically reduced by viable cells to yield a soluble, colored formazan product measurable by conventional colorimetric techniques. This simple microassay minimizes the number of plate manipulations typically required with other assay methods and, coupled with computerized data collection and analysis, facilitates large-scale screening of agents for potential antiviral activity. To support and enhance the discovery of new anti-HIV-1 agents, the National Cancer Institute is offering investigators worldwide the opportunity to submit new candidate agents for anti-HIV-1 screening with this method.
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Citations
Cited by
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Antiviral activity of Plantago major extracts and related compounds in vitro
Chiang, L. C., Chiang, W., Chang, M. Y., Ng, L. T., & Lin, C. C. (2002) · Antiviral Research · doi:10.1016/s0166-3542(02)00007-4 · Cited by 334
Buttke, T. M., McCubrey, J. A., & Owen, T. C. (1993) · Journal of Immunological Methods · doi:10.1016/0022-1759(93)90092-l · Cited by 323
An evaluation of the antioxidant and antiviral action of extracts of rosemary and provençal herbs
Aruoma, O. I., Spencer, J. P. E., Rossi, R., Aeschbach, R., Khan, A., Mahmood, N., Munoz, A., Murcia, A., Butler, J., & Halliwell, B. (1996) · Food and Chemical Toxicology · doi:10.1016/0278-6915(96)00004-x · Cited by 288
Metadata
- Authors
- Weislow, O. S., Kiser, R., Fine, D. L., Bader, J., Shoemaker, R. H., & Boyd, M. R.
- Venue
- JNCI: Journal of the National Cancer Institute
- Publisher
- Oxford University Press (OUP)
- Published
- 1989-04-19
- Volume
- 81
- Issue
- 8
- Pages
- 577-586
- ISSN
- 0027-8874 (ISSN-L)
- 1460-2105
- OpenAlex
- W1976683819
- Cited by
- 685
- References
- 0
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: New Soluble-Formazan Assay for HIV-1 Cytopathic Effects: Application to High-Flux Screening of Synthetic and Natural Products for AIDS-Antiviral Activity. https://app.cochat.ai/papers/10.1093/jnci/81.8.577
- The paper
- Weislow, O. S., Kiser, R., Fine, D. L., Bader, J., Shoemaker, R. H., & Boyd, M. R. (1989). New Soluble-Formazan Assay for HIV-1 Cytopathic Effects: Application to High-Flux Screening of Synthetic and Natural Products for AIDS-Antiviral Activity. JNCI: Journal of the National Cancer Institute. https://doi.org/10.1093/jnci/81.8.577
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
