The DOI matches its Crossref record; 58 of the 59 references it cites were found in a registry or an index.
Telomerase Repressor Sequences on Chromosome 3 and Induction of Permanent Growth Arrest in Human Breast Cancer Cells
Abstract
BACKGROUND: Activation of the enzyme telomerase, which has been associated with cellular immortality, may constitute a key step in the development of human cancer. Telomerase is repressed in most normal human somatic cells. This study was conducted, using a genetic complementation approach, with the aim of identifying and mapping the genes responsible for repressing telomerase and, simultaneously, to establish the effect of experimentally induced telomerase repression on human tumor cell growth. METHODS: Individual human chromosomes isolated from normal diploid cells and tagged with bacterial antibiotic resistance genes (for later selection) were introduced into cells of the human breast carcinoma cell line 21NT by means of microcell transfer. Selected hybrid clones were screened for telomerase activity by use of the polymerase chain reaction-based telomere repeat amplification protocol (TRAP) assay, and the proliferative fate of the hybrid clones was determined. Regions of the introduced chromosomes associated with telomerase repression were mapped using segregant hybrids and a deletion analysis that employed microsatellite DNA markers. RESULTS: Strong repression of telomerase was observed following transfer of human chromosome 3 into 21NT cells but not after transfer of chromosomes 8, 12, or 20. The vast majority of hybrid clones with repressed telomerase entered permanent growth arrest after 10-18 population doublings. Deletion analysis of nonrepressed segregant monochromosome 3 hybrids indicated two regions on the short arm of chromosome 3 (3p21.3-p22 and 3p12-21.1) where telomerase regulator genes may be located. CONCLUSIONS: Telomerase in human breast cancer cells is efficiently repressed by a gene or genes on normal human chromosome 3p, and this repression is associated with permanent growth arrest of the tumor cells.
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“Several normal human chromosomes, including chromosomes 3, 4, 6, 7, 10, and 17, have been shown to repress telomerase activity in some but not all cancer cells.(77)(78)(79)(80)(81)(82)(83)(84)(85)”
From Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers (2008) · cites this paper as [79]
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Metadata
- Authors
- Cuthbert, A. P., Bond, J., Trott, D. A., Gill, S., Broni, J., Marriott, A., Khoudoli, G., Parkinson, E. K., Cooper, C. S., & Newbold, R. F.
- Affiliations
- Brunel University of London (GB)
- Corresponding author
- Cuthbert, A. P.
- Venue
- JNCI Journal of the National Cancer Institute
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-01-06
- Volume
- 91
- Issue
- 1
- Pages
- 37-45
- ISSN
- 0027-8874 (ISSN-L)
- 1460-2105
- OpenAlex
- W2102936230
- Cited by
- 114
- References
- 59
- Citations by year
- 2024: 1 · 2021: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 1
- FWCI
- 13.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- JNCI Journal of the National Cancer Institute (publishedVersion)
- PubMed (publishedVersion)
- Brunel University Research Archive (BURA) (Brunel University London) (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- telomerase, chromosome 3, breast cancer, growth arrest, gene complementation, chromosome 3p, telomere repeat amplification protocol
- MeSH terms
- Adult, Breast Neoplasms, Carcinoma, Intraductal, Noninfiltrating, Cell Division, Cell Fusion, Chromosomes, Chromosomes, Human, Pair 3, Female, Growth Inhibitors, Humans, Hybrid Cells, Neoplasm Proteins, Repressor Proteins, Tumor Cells, Cultured, Gene Expression Regulation, Enzymologic, Polymerase Chain Reaction, Telomere, In Situ Hybridization, Fluorescence, Gene Transfer Techniques, Telomerase
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