Barley transcription factor HvNLP2 mediates nitrate signaling and affects nitrogen use efficiency
Abstract
Plants have evolved complex mechanisms to adapt to the changing nitrogen levels in the environment. In Arabidopsis, more than a dozen nitrate signaling regulatory genes have been characterized, including the NODULE INCEPTION-LIKE PROTEIN (AtNLP) genes, which play essential roles in nitrate signaling. However, whether NLP genes in the Triticeae crops are involved in nitrate regulation and nitrogen use efficiency (NUE) remains unknown. Here, we isolated a barley (Hordeum vulgare L.) mutant, hvnlp2-1, from a TILLING (Targeting Local Lesions IN Genomes) population and constructed two RNAi lines, hvnlp2-2 and hvnlp2-3, to study the function of HvNLP2. The expression of the nitrate-responsive genes was substantially inhibited after nitrate treatment in the hvnlp2 mutants, indicating that HvNLP2 controls nitrate signaling. Nitrate content was significantly higher in the hvnlp2 mutants, which may result from the decreased assimilation of nitrogen caused by reduced nitrate reductase activity and expression of nitrate assimilatory genes. HvNLP2 is localized to the nucleus in the presence of nitrate. Further investigation showed that HvNLP2 binds to and activates the nitrate-responsive cis-elements. Moreover, hvnlp2 exhibited reduced biomass, seed yield, and NUE. Therefore, HvNLP2 controls nitrate signaling and plays an important role in NUE.
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It is estimated that only 30%-40% of the current year's applied N fertilizer is taken up by crops, with the remainder remaining in the soil or lost out of the cropping system into the air and water.
“It is estimated that only 30%-40% of the current year's applied N fertilizer is taken up by crops, with the remainder remaining in the soil or lost out of the cropping system into the air and water (Yan et al., 2020;He et al., 2021;Gao et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Gao et al., 2022
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- Authors
- Gao, Y., Quan, S., Lyu, B., Tian, T., Liu, Z., Nie, Z., Qi, S., Jia, J., Shu, J., Groot, E., Wu, J., & Wang, Y.
- Affiliations
- State Key Laboratory of Crop Biology (CN)
- Shandong Agricultural University (CN)
- Shangdong Agriculture and Engineering University (CN)
- Corresponding author
- Wu, Jiajie
- Wang, Yong
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2021-05-29
- Volume
- 73
- Issue
- 3
- Pages
- 770-783
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W3164985416
- Cited by
- 32
- References
- 63
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 6 · 2023: 10 · 2022: 3
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Experimental Botany (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Key Research and Development Program of China (2016YFD0100701)
- National Natural Science Foundation of China (31970270, 31902100)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nitrogen and Sulfur Effects on Brassica
- Keywords
- nitrate signaling, nitrogen use efficiency, barley, nitrate reductase, nitrate assimilation, transcription factors, seed yield, Triticeae
- MeSH terms
- Hordeum, Nitrates, Nitrogen, Transcription Factors, Crops, Agricultural
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