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Cited by 348Plant Surface Properties and TreatmentsPlant Stress Responses and ToleranceLipid metabolism and biosynthesis

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

Tomato fruit cuticular waxes and their effects on transpiration barrier properties: functional characterization of a mutant deficient in a very-long-chain fatty acid -ketoacyl-CoA synthase

Vogg, G., Fischer, S., Leide, J., Emmanuel, E., Jetter, R., Levy, A. A., Riederer, M.·2004·Journal of Experimental Botany
DOI 10.1093/jxb/erh149
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Abstract

Cuticular waxes play a pivotal role in limiting transpirational water loss across the plant surface. The correlation between the chemical composition of the cuticular waxes and their function as a transpiration barrier is still unclear. In the present study, intact tomato fruits (Lycopersicon esculentum) are used, due to their astomatous surface, as a novel integrative approach to investigate this composition- function relationship: wax amounts and compositions of tomato were manipulated before measuring unbiased cuticular transpiration. First, successive mechanical and extractive wax-removal steps allowed the selective modification of epi- and intracuticular wax layers. The epicuticular film consisted exclusively of very-long-chain aliphatics, while the intracuticular compartment contained large quantities of pentacyclic triterpenoids as well. Second, applying reverse genetic techniques, a loss-of-function mutation with a transposon insertion in a very-long-chain fatty acid elongase beta-ketoacyl-CoA synthase was isolated and characterized. Mutant leaf and fruit waxes were deficient in n-alkanes and aldehydes with chain lengths beyond C30, while shorter chains and branched hydrocarbons were not affected. The mutant fruit wax also showed a significant increase in intracuticular triterpenoids. Removal of the epicuticular wax layer, accounting for one-third of the total wax coverage on wild-type fruits, had only moderate effects on transpiration. By contrast, reduction of the intracuticular aliphatics in the mutant to approximately 50% caused a 4-fold increase in permeability. Hence, the main portion of the transpiration barrier is located in the intracuticular wax layer, largely determined by the aliphatic constituents, but modified by the presence of triterpenoids, whereas epicuticular aliphatics play a minor role.

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Citations

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Research works printed but not linked: 5

  1. 2002, LEBENSMITTELCHEMIE, 56, 13
  2. 1990, Planta, 180, 147
  3. 1997, Genetics, 134, 571
  4. 1994, EUROPEAN BIOPHYSICAL JOURNAL, 23, 59
  5. 1990, Planta, 180, 154

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Metadata

Authors
Vogg, G., Fischer, S., Leide, J., Emmanuel, E., Jetter, R., Levy, A. A., & Riederer, M.
Affiliations
University of Würzburg (DE)
Corresponding author
Vogg, G.
ORCID
Venue
Journal of Experimental Botany
Publisher
Oxford University Press (OUP)
Published
2004-05-07
Volume
55
Issue
401
Pages
1401-1410
ISSN
  • 0022-0957 (ISSN-L)
  • 1460-2431
DOI
10.1093/jxb/erh149
OpenAlex
W2158715711
Cited by
348
References
44
Citations by year
2026: 5 · 2025: 13 · 2024: 15 · 2023: 24 · 2022: 27 · 2021: 25 · 2020: 27 · 2019: 21 · 2018: 9 · 2017: 22 · 2016: 12 · 2015: 20 · 2014: 18 · 2013: 20 · 2012: 18
FWCI
12.90
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Experimental Botany (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Surface Properties and Treatments
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
cuticular wax, tomato fruit, very long chain fatty acids, β-ketoacyl-CoA synthase, transposon mutants, epicuticular wax, pentacyclic triterpenes, n-alkanes, wax removal
MeSH terms
Acyltransferases, Base Sequence, Cell Membrane Permeability, Fruit, Molecular Sequence Data, Mutation, Water, Waxes, 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase, Plant Leaves, Plant Transpiration, Solanum lycopersicum, Plant Epidermis, Arabidopsis Proteins

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