Over-expression of a tomato N-acetyl-L-glutamate synthase gene (SlNAGS1) in Arabidopsis thaliana results in high ornithine levels and increased tolerance in salt and drought stresses
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A single copy of the N-acetyl-L-glutamate synthase gene (SlNAGS1) has been isolated from tomato. The deduced amino acid sequence consists of 604 amino acids and shows a high level of similarity to the predicted Arabidopsis NAGS1 and NAGS2 proteins. Furthermore, the N-terminus ArgB domain and the C-terminus ArgA domain found in SlNAGS1 are similar to the structural arrangements that have been reported for other predicted NAGS proteins. SlNAGS1 was expressed at high levels in all aerial organs, and at basic levels in seeds, whereas it was not detected at all in roots. SlNAGS1 transcript accumulation was noticed transiently in tomato fruit at the red-fruit stage. In addition, an increase of SlNAGS1 transcripts was detected in mature green tomato fruit within the first hour of exposure to low oxygen concentrations. Transgenic Arabidopsis plants have been generated expressing the SlNAGS1 gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Three homozygous transgenic lines expressing the transgene (lines 1-7, 3-8, and 6-5) were evaluated further. All three transgenic lines showed a significant accumulation of ornithine in the leaves with line 3-8 exhibiting the highest concentration. The same lines demonstrated higher germination ability compared to wild-type (WT) plants when subjected to 250 mM NaCl. Similarly, mature plants of all three transgenic lines displayed a higher tolerance to salt and drought stress compared to WT plants. Under most experimental conditions, transgenic line 3-8 performed best, while the responses obtained from lines 1-7 and 6-5 depended on the applied stimulus. To our knowledge, this is the first plant NAGS gene to be isolated, characterized, and genetically modified.
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Better salt tolerance can be conferred through expression of glycinebetaine and polyamines in terrestrial plants.
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Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684 · see citing works
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302 · see citing works
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Tamura, K., Dudley, J. T., Nei, M., & Kumar, S. (2007) · Molecular Biology and Evolution · doi:10.1093/molbev/msm092 · Cited by 28,734 · see citing works
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS
Arnon, D. I. (1949) · PLANT PHYSIOLOGY · doi:10.1104/pp.24.1.1 · Cited by 22,508 · see citing works
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014 · see citing works
Church, G. M., & Gilbert, W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.7.1991 · Cited by 8,213 · see citing works
Ashburner, M. (1989) · Cold Spring Harbor Laboratory eBooks · Cited by 6,739 · see citing works
Potential biochemical indicators of salinity tolerance in plants
Ashraf, M., & Harris, P. J. C. (2003) · Plant Science · doi:10.1016/j.plantsci.2003.10.024 · Cited by 2,068 · see citing works
Emanuelsson, O., Nielsen, H., & von HEIJNE, G. (1999) · Protein Science · doi:10.1110/ps.8.5.978 · Cited by 1,790 · see citing works
Hoekema, A., Hirsch, P. R., Hooykaas, P. J. J., et al. (1983) · Nature · doi:10.1038/303179a0 · Cited by 1,764 · see citing works
Proline biosynthesis and osmoregulation in plants
Delauney, A. J., & Verma, D. P. S. (1993) · The Plant Journal · doi:10.1046/j.1365-313x.1993.04020215.x · Cited by 1,758 · see citing works
An evaluation of noninvasive methods to estimate foliar chlorophyll content
Richardson, A. D., Duigan, S. P., & Berlyn, G. P. (2002) · New Phytologist · doi:10.1046/j.0028-646x.2001.00289.x · Cited by 1,158 · see citing works
Plant Molecular Biology Manual
Gelvin, S. B., Schilperoort, R. A., & Verma, D. P. S. (1990) · Springer eBooks · doi:10.1007/978-94-017-6953-2 · Cited by 1,064 · see citing works
The PROSITE database, its status in 2002
Falquet, L. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.1.235 · Cited by 1,001 · see citing works
CDD: a conserved domain database for interactive domain family analysis
Marchler‐Bauer, A., Anderson, J. B., Derbyshire, M. K., DeWeese-Scott, C., Gonzales, N. R., Gwadz, M., Hao, L., He, S., Hurwitz, D. I., Jackson, J. D., et al. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl951 · Cited by 851 · see citing works
Lea, P. J., Sodek, L., Parry, M. A. J., Shewry, P. R., & Halford, N. G. (2006) · Annals of Applied Biology · doi:10.1111/j.1744-7348.2006.00104.x · Cited by 667 · see citing works
Capell, T., Bassié, L., & Christou, P. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0306974101 · Cited by 604 · see citing works
Nitrogen Containing Compounds and Adaptation of Plants to Salinity Stress
Mansour, M. M. F. (2000) · Biologia Plantarum · doi:10.1023/a:1002873531707 · Cited by 574 · see citing works
Genes, enzymes and regulation of arginine biosynthesis in plants
Slocum, R. D. (2005) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2005.06.007 · Cited by 297 · see citing works
Mazzucotelli, E., Mastrangelo, A. M., Crosatti, C., Guerra, D., Stanca, A. M., & Cattivelli, L. (2008) · Plant Science · doi:10.1016/j.plantsci.2008.02.005 · Cited by 277 · see citing works
Involvement of polyamines in the drought resistance of rice
Yang, J., Zhang, J., Liu, K., Wang, Z., & Liu, L. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm032 · Cited by 262 · see citing works
Akashi, K., Miyake, C., & Yokota, A. (2001) · FEBS Letters · doi:10.1016/s0014-5793(01)03123-4 · Cited by 255 · see citing works
Roosens, N. H. C., Thu, T. T. M., MINARSIH, H., & Jacobs, M. (1998) · PLANT PHYSIOLOGY · doi:10.1104/pp.117.1.263 · Cited by 234 · see citing works
A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis
Wadsworth, G. J., Redinbaugh, M. G., & Scandalios, J. G. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90443-5 · Cited by 215 · see citing works
Citrulline and DRIP-1 Protein (ArgE Homologue) in Drought Tolerance of Wild Watermelon
Yokota, A. (2002) · Annals of Botany · doi:10.1093/aob/mcf074 · Cited by 181 · see citing works
N-Acetylglutamate and its changing role through evolution
Caldovic, L., & Tuchman, M. (2003) · Biochemical Journal · doi:10.1042/bj20030002 · Cited by 163 · see citing works
Genetic Architecture of NaCl Tolerance in Arabidopsis
Quesada, V., Garcı́a-Martı́nez, S., Piqueras, P., et al. (2002) · PLANT PHYSIOLOGY · doi:10.1104/pp.006536 · Cited by 163 · see citing works
Kawasaki, S., Miyake, C., Kohchi, T., et al. (2000) · Plant and Cell Physiology · doi:10.1093/pcp/pcd005 · Cited by 152 · see citing works
An, G., Ebert, P. R., Mitra, A., & Ha, S. B. (1988) · Springer eBooks · doi:10.1007/978-94-017-5294-7_3 · Cited by 131 · see citing works
Heinrich, A., Mani, M., Ruppert, U., & Forchhammer, K. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04058.x · Cited by 130 · see citing works
Boggio, S. B., Palatnik, J. F., Heldt, H. W., et al. (2000) · Plant Science · doi:10.1016/s0168-9452(00)00342-3 · Cited by 125 · see citing works
Piao, H. L., Lim, J. H., Kim, S. J., Cheong, G., & Hwang, I. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01099.x · Cited by 124 · see citing works
Chen, Y. M., Ferrar, T. S., Lohmeier‐Vogel, E. M., MORRICE, N. A., Mizuno, Y., Berenger, B. M., Ng, K. K.-S., Muench, D. G., & Moorhead, G. B. G. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m510945200 · Cited by 114 · see citing works
Smith, C. J. S., Slater, R. J., & Grierson, D. S. (1986) · Planta · doi:10.1007/bf00407014 · Cited by 107 · see citing works
Diallinas, G., Pateraki, I., Sanmartín, M., et al. (1997) · Plant Molecular Biology · doi:10.1023/a:1005851527227 · Cited by 98 · see citing works
Self-citationInteraction of N-Acetylglutamate Kinase with a PII-Like Protein in Rice
Sugiyama, K., Hayakawa, T., Kudo, T., et al. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pch199 · Cited by 81 · see citing works
Ornithine biosynthesis, and arginine biosynthesis and degradation in plant cells
Shargool, D., Jain, J. C., & McKay, G. (1988) · Phytochemistry · doi:10.1016/0031-9422(88)80404-7 · Cited by 80 · see citing works
The regulatory PII protein controls arginine biosynthesis in Arabidopsis
Ferrario, S., Besin, E., Pichon, O., et al. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.02.075 · Cited by 80 · see citing works
Ramón‐Maiques, S., Fernández-Murga, M. L., Gil-Ortiz, F., et al. (2005) · Journal of Molecular Biology · doi:10.1016/j.jmb.2005.11.079 · Cited by 74 · see citing works
Structural Basis for the Regulation of N-Acetylglutamate Kinase by PII in Arabidopsis thaliana
Mizuno, Y., Moorhead, G. B. G., & Ng, K. K.-S. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m707127200 · Cited by 63 · see citing works
Loulakakis, K. A., Roubelakis–Angelakis, K. A., & Kanellis, A. K. (1994) · PLANT PHYSIOLOGY · doi:10.1104/pp.106.1.217 · Cited by 60 · see citing works
Self-citationPatil, S. S., Kolattukudy, P. E., & Dimond, A. E. (1970) · PLANT PHYSIOLOGY · doi:10.1104/pp.46.5.752 · Cited by 59 · see citing works
Rajagopal, B. S., DePonte, J., Tuchman, M., & Malamy, M. H. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.5.1805-1811.1998 · Cited by 56 · see citing works
Mode of Action of the Toxin from Pseudomonas phaseolicola
Patil, S. S., Tam, L. Q., & Sakai, W. S. (1972) · PLANT PHYSIOLOGY · doi:10.1104/pp.49.5.803 · Cited by 55 · see citing works
Marvil, D. K., & Leisinger, T. (1977) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40387-5 · Cited by 49 · see citing works
Khuhawar, M. Y., & Rajper, A. D. (2003) · Journal of Chromatography B · doi:10.1016/s1570-0232(03)00062-x · Cited by 41 · see citing works
Qu, Q., Morizono, H., Shi, D., et al. (2007) · BMC Biochemistry · doi:10.1186/1471-2091-8-4 · Cited by 28 · see citing works
Putative Nitrogen Sensing Systems in Higher Plants
Lam, H., Chiao, Y. A., Li, M., et al. (2006) · Journal of Integrative Plant Biology · doi:10.1111/j.1744-7909.2006.00313.x · Cited by 24 · see citing works
Sancho‐Vaello, E., Fernández-Murga, M. L., & Rubio, V. (2008) · FEBS Letters · doi:10.1016/j.febslet.2008.02.060 · Cited by 18 · see citing works
Antisenescence properties of guazatine in osmotically stressed oat leaves
Capell, T., Campos, J. L., & Tiburcio, A. F. (1993) · Phytochemistry · doi:10.1016/0031-9422(93)85205-6 · Cited by 17 · see citing works
Compartmentation studies on enzymes of ornithine biosynthesis in plant cells
Jain, J. C., Shargool, P. D., & Chung, S.-M. (1987) · Plant Science · doi:10.1016/0168-9452(87)90215-9 · Cited by 14 · see citing works
Khuhawar, M. Y., & Rajper, A. D. (2003) · Chromatographia · doi:10.1365/s10337-003-0072-0 · Cited by 6 · see citing works
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Biofuels from algae: Challenges and potential
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- Authors
- Kalamaki, M. S., Alexandrou, D., Lazari, D., Merkouropoulos, G., Fotopoulos, V., Pateraki, I., Aggelis, A., Carrillo-López, A., Rubio-Cabetas, M. J., & Kanellis, A. K.
- Affiliations
- Aristotle University of Thessaloniki (GR)
- Corresponding author
- Kanellis, Angelos K.
- ORCID
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-04
- Volume
- 60
- Issue
- 6
- Pages
- 1859-1871
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- PMC
- PMC2671631
- OpenAlex
- W2115848425
- Cited by
- 97
- References
- 52
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 7 · 2023: 4 · 2022: 5 · 2021: 8 · 2020: 2 · 2019: 6 · 2018: 3 · 2017: 6 · 2016: 7 · 2015: 7 · 2014: 4 · 2013: 9 · 2012: 3
- FWCI
- 6.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Journal of Experimental Botany (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Ktisis at Cyprus University of Technology (Cyprus University of Technology) (submittedVersion, cc-by-nc)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
- Keywords
- tomato, salt tolerance, drought tolerance, transgenic Arabidopsis, urea cycle
- MeSH terms
- Amino Acid Sequence, Molecular Sequence Data, Ornithine, Plant Proteins, Sodium Chloride, Gene Expression, Arabidopsis, Gene Expression Regulation, Plant, Solanum lycopersicum, Amino-Acid N-Acetyltransferase, Salt Tolerance, Droughts
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