Virus-induced gene silencing of Arabidopsis thaliana gene homologues in wheat identifies genes conferring improved drought tolerance
Open accessAbstract
In a non-model staple crop like wheat (Triticum aestivumI L.), functional validation of potential drought stress responsive genes identified in Arabidopsis could provide gene targets for breeding. Virus-induced gene silencing (VIGS) of genes of interest can overcome the inherent problems of polyploidy and limited transformation potential that hamper functional validation studies in wheat. In this study, three potential candidate genes shown to be involved in abiotic stress response pathways in Arabidopsis thaliana were selected for VIGS experiments in wheat. These include Era1 (enhanced response to abscisic acid), Cyp707a (ABA 8'-hydroxylase), and Sal1 (inositol polyphosphate 1-phosphatase). Gene homologues for these three genes were identified in wheat and cloned in the viral vector barley stripe mosaic virus (BSMV) in the antisense direction, followed by rub inoculation of BSMV viral RNA transcripts onto wheat plants. Quantitative real-time PCR showed that VIGS-treated wheat plants had significant reductions in target gene transcripts. When VIGS-treated plants generated for Era1 and Sal1 were subjected to limiting water conditions, they showed increased relative water content, improved water use efficiency, reduced gas exchange, and better vigour compared to water-stressed control plants inoculated with RNA from the empty viral vector (BSMV0). In comparison, the Cyp707a-silenced plants showed no improvement over BSMV0-inoculated plants under limited water condition. These results indicate that Era1 and Sal1 play important roles in conferring drought tolerance in wheat. Other traits affected by Era1 silencing were also studied. Delayed seed germination in Era1-silenced plants suggests this gene may be a useful target for developing resistance to pre-harvest sprouting.
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VIGS has been used to functionally analyse the Triticum aestivum enhanced response to abscisic acid 1 (TaEra1) and basic transcription factor 3 (TaBTF3) genes in wheat, and inwardly rectifying potassium (K+) channels HvAKT1 and HvAKT2, HVA1, and Hv dehydrin 6 (HvDhn6) genes in barley for drought tolerance.
“VIGS has been used to functionally analyse the Triticum aestivum enhanced response to abscisic acid 1 (TaEra1) and basic transcription factor 3 (TaBTF3) genes in wheat, and inwardly rectifying potassium (K + ) channels HvAKT1 and HvAKT2, HVA1, and Hv dehydrin 6 (HvDhn6) genes in barley for drought tolerance (Liang et al., 2012;Kang et al., 2013;Manmathan et al., 2013;Feng et al., 2020a).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Manmathan et al., 2013
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Protein Farnesylation Takes Part in Arabidopsis Seed Development
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RetractedCloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice
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Applying Genomics Resources to Accelerate the Development of Climate Resilient Crops
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Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors
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- Authors
- Manmathan, H., Shaner, D., Snelling, J., Tisserat, N., & Lapitan, N.
- Affiliations
- Colorado State University (US)
- Agricultural Research Service (US)
- Corresponding author
- Lapitan, Nora
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-01-30
- Volume
- 64
- Issue
- 5
- Pages
- 1381-1392
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- PMC
- PMC3598424
- OpenAlex
- W2118469923
- Cited by
- 99
- References
- 46
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 7 · 2023: 6 · 2022: 8 · 2021: 7 · 2020: 10 · 2019: 8 · 2018: 10 · 2017: 4 · 2016: 6 · 2015: 3 · 2014: 11 · 2013: 7
- FWCI
- 10.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc
- Locations
- Journal of Experimental Botany (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture (2009‐34205‐19960, 2010-34205-21350)
- Colorado State University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- virus-induced gene silencing, wheat, drought tolerance, CYP707A, abscisic acid, barley stripe mosaic virus, abiotic stress response, pre-harvest sprouting, seed germination, water use efficiency, homologous genes, functional validation
- MeSH terms
- Adaptation, Physiological, Dehydration, Gases, Mosaic Viruses, Phenotype, Plant Proteins, RNA, Messenger, Seeds, Sequence Homology, Nucleic Acid, Triticum, Genes, Plant, Arabidopsis, Gene Expression Regulation, Plant, Germination, Gene Silencing, Plants, Genetically Modified, Plant Stomata, Droughts, Real-Time Polymerase Chain Reaction
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