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Cited by 160

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Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae

Open access
Macreadie, Ian G., Novitski, Charles E., Maxwell, Ronald J., John, Ulrik, Ooi, Beng-Guat, McMullen, Gabrielle L., Lukins, H.B., Linnane, Anthony W., Nagley, Phillip·1983·Nucleic Acids Research

Abstract

A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit- mutants and revertants. Evidence is presented that the aap1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein.

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Crossref · 10.1093/nar/11.13.4435

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Metadata

Authors
Macreadie, I. G., Novitski, C. E., Maxwell, R. J., John, U., Ooi, B.-G., McMullen, G. L., Lukins, H. B., Linnane, A. W., & Nagley, P.
Venue
Nucleic Acids Research
Published
1983
Volume
11
Issue
13
Pages
4435-4451
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W1979275335
Cited by
160
References
1
FWCI
7.82
Type
journal article
Language
en
Open access
green

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Macreadie, I. G., Novitski, C. E., Maxwell, R. J., John, U., Ooi, B.-G., McMullen, G. L., Lukins, H. B., Linnane, A. W., & Nagley, P. (1983). Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae. Nucleic Acids Research. https://doi.org/10.1093/nar/11.13.4435

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