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Expression of recombinant plasmids in mammalian cells Is enhanced by sodium butyrate
Open accessAbstract
We have studied the effects of sodium butyrate on DNA-mediated gene transfer in an effort to investigate interrelationships between chromatin structure and expression of recombinant plasmids. Our results demonstrate that butyrate affects the early stages of gene activity following DNA uptake at least two levels. First, the number of cells able to express foreign DNA increases from 10% to up to 40%. Second, there is an increase in enhancer-dependent transcription, approximately 30 fold in HeLa cells, involving the SV40 early promoter. Stable transformation efficiencies increase to 4% and 10% in HeLa S3 and monkey kidney CV-1 cells, respectively. Finally, expression of integrated recombinant plasmid genes is reinducible by a second treatment five weeks after initial exposure to this agent.
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Cites
In vivo sequence requirements of the SV40 early promoter region
Benoist, C., & Chambon, P. (1981) · Nature · doi:10.1038/290304a0 · Cited by 1,074
Cited by
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Wurm, F. M. (2004) · Nature Biotechnology · doi:10.1038/nbt1026 · Cited by 2,032
Sladek, F. M., Zhong, W. M., Lai, E., & Darnell, J. E. (1990) · Genes & Development · doi:10.1101/gad.4.12b.2353 · Cited by 1,027
Röper, K., Corbeil, D., & Huttner, W. B. (2000) · Nature Cell Biology · doi:10.1038/35023524 · Cited by 555
Expression of Recombinant Antibodies
Frenzel, A., Hust, M., & Schirrmann, T. (2013) · Frontiers in Immunology · doi:10.3389/fimmu.2013.00217 · Cited by 353
Teufel, M., Saudek, V., Ledig, J.-P., Bernhardt, A., Boularand, S., Carreau, A., Cairns, N. J., Carter, C., Cowley, D. J., Duverger, D., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m209764200 · Cited by 346
Metadata
- Authors
- Gorman, C. M., Howard, B. H., & Reeves, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1983
- Volume
- 11
- Issue
- 21
- Pages
- 7631-7648
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2098551568
- Cited by
- 367
- References
- 1
- FWCI
- 5.98
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gorman, C. M., Howard, B. H., & Reeves, R. (1983). Expression of recombinant plasmids in mammalian cells Is enhanced by sodium butyrate. Nucleic Acids Research. https://doi.org/10.1093/nar/11.21.7631
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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