In vivo sequence requirements of the SV40 early promoter region
Abstract
To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, we have constructed several deletion mutants in the promoter region. The TATA box region is apparently involved in fixing initiation precisely within a narrow area, but is dispensable for gene expression, while the sequences located more than 150 base pairs upstream are indispensable.
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A 72-bp repeat sequence element in SV40 is an enhancing sequence capable of vastly upregulating gene expression from a plasmid upon transfection into mammalian cells.
“Subsequent landmark experiments by the groups of Walter Schaffner [4] and Pierre Chambon [5] confirmed that a 72-bp repeat sequence element in SV40 [8, 9] was an “enhancing” sequence, capable of vastly upregulating gene expression from a plasmid upon transfection into mammalian cells.”
From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [8]
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- Authors
- Benoist, C., & Chambon, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Nature
- Published
- 1981-03
- Volume
- 290
- Issue
- 5804
- Pages
- 304-310
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2019590714
- Cited by
- 1,074
- References
- 0
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 5 · 2023: 9 · 2022: 9 · 2021: 11 · 2020: 9 · 2019: 11 · 2018: 8 · 2017: 7 · 2016: 5 · 2015: 7 · 2014: 2 · 2013: 5 · 2012: 3
- FWCI
- 66.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Polyomavirus and related diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- SV40 early promoter, transcription initiation, TATA box, promoter deletion mutants, upstream sequences, viral gene expression, sequence requirements
- MeSH terms
- Antigens, Neoplasm, Antigens, Viral, Antigens, Viral, Tumor, Base Sequence, DNA, Viral, Gene Expression Regulation, Mutation, Operon, Simian virus 40, Transcription, Genetic
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