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pEMBL: a new family of single stranded plasmids
Open accessAbstract
We have constructed a series of plasmids, the pEMBL family, characterized by the presence of 1) the bla gene as selectable marker, 2) a short segment coding for the alpha-peptide of beta-galactosidase and containing a multiple cloning sites polylinker, 3) the intragenic region of phage F1. pEMBL plasmids have the property of being encapsidated as single stranded DNA, upon superinfection with phage F1. These vectors have been used successfully for DNA sequencing with the dideoxy-method, and can be used for any other purpose for which M13 derivatives are used. However, the pEMBL plasmids have the advantage of being smaller than M13 vectors, and the purification of the DNA is simpler. In addition, and most importantly, long inserts have a higher stability in pEMBL plasmids than M13 vectors.
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Cited by
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Poole, S. J., Kauvar, L. M., Drees, B., & Kornberg, T. (1985) · Cell · doi:10.1016/0092-8674(85)90306-x · Cited by 721
Isberg, R. R., Voorhis, D. L., & Falkow, S. (1987) · Cell · doi:10.1016/0092-8674(87)90335-7 · Cited by 597
New pUC-derived cloning vectors with different selectable markers and DNA replication origins
Jeffrey, V., & Joachim, M. (1991) · Gene · doi:10.1016/0378-1119(91)90365-i · Cited by 494
Ligtenberg, M. J., Vos, H. L., Gennissen, A. M., & Hilkens, J. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39399-8 · Cited by 369
Lipinska, B., Fayet, O., Baird, L., & Georgopoulos, C. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.3.1574-1584.1989 · Cited by 342
Metadata
- Authors
- Dente, L., Cesareni, G., & Cortese, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1983
- Volume
- 11
- Issue
- 6
- Pages
- 1645-1655
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2008936546
- Cited by
- 1,164
- References
- 0
- FWCI
- 54.26
- Type
- journal article
- Language
- en
- Open access
- green
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- Dente, L., Cesareni, G., & Cortese, R. (1983). pEMBL: a new family of single stranded plasmids. Nucleic Acids Research. https://doi.org/10.1093/nar/11.6.1645
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