Site specific insertion of a type I rDNA dement into a unique sequence in the Drosophila melanogaster genome
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We describe a cloned segment of unique DNA from the Oregon R strain of Drosophila melanogaster that contains a short type I insertion of the kind principally found within rDNA. The predominant type I rDNA insertion is 5kb in length, but there are also a co-terminal sub-set of shorter type I elements that share a common right hand junction with the rDNA. The insertion that we now describe is another member of this sub-set. The right hand junction of the type I sequence with the unique DNA is identical to the right hand junction of the type I sequences with rDNA. There is no significant feature within the insertion sequence that could have determined the position of the left junction with the sequence into which it is inserted. Like the corresponding short type I insertions in rDNA, the insertion into the unique DNA is flanked on both sides by a duplicated sequence, which in this case is 10 base pairs long. The cloning of a sequence corresponding to the uninterrupted unique location was facilitated by the observation that the Karsnas strain of D. melanogaster contains only uninterrupted sequences of this kind. The duplicated sequence at the target site for the insertion is only present as a single copy in the uninterrupted DNA. The sequence of the target site for the insertion (ACTGTTCT) in the unique segment shows a striking homology to the target in rDNA (ACTGTCCC).
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R2 elements are present exclusively at the rDNA locus, whereas R1 insertions have also been found in other regions of the genome.
“R2 elements are of particular interest because they are present exclusively at the rDNA locus, whereas R1 insertions have also been found in other regions of the genome (35–38).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [37]
The Introduction says type II insertions occur in rDNA units, but does not establish that they occur exclusively at the rDNA locus. It describes type I elements outside rDNA, and the Discussion reports a type I insertion in a unique genomic sequence. Thus the R1 portion is supported, but the exclusivity claim for R2 is not established by this copy. (LLM, conf=0.87)
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Research works printed but not linked: 1
- Roiha, H., Read, C.A., Browne, M.J. and Glover, D.M. (1983). E.M.B.O. Journal 2, 721-726
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Guerrero, P. A., & Maggert, K. A. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016401 · Cited by 51 · see citing works
Fefelova, E. A., Pleshakova, I. M., Mikhaleva, E. A., Pirogov, S. A., Poltorachenko, V. A., Abramov, Y. A., Romashin, D. D., Shatskikh, A. S., Blokh, R. S., Gvozdev, V. A., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1276 · Cited by 15 · see citing works
Raje, H. S., Lieux, M. E., & DiMario, P. J. (2018) · Transcription · doi:10.1080/21541264.2018.1506682 · Cited by 13 · see citing works
- Authors
- Browne, M. J., Read, C. A., Roiha, H., & Glover, D. M.
- Affiliations
- Imperial College London (GB)
- ORCID
- Glover, David M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1984
- Volume
- 12
- Issue
- 23
- Pages
- 9111-9122
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC320441
- OpenAlex
- W2035870751
- Cited by
- 19
- References
- 0
- Citations by year
- 2025: 2 · 2021: 1 · 2018: 1 · 2013: 1
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion, cc-by-nc)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomic variations and chromosomal abnormalities
- Keywords
- Drosophila melanogaster, ribosomal DNA, site-specific insertion
- MeSH terms
- Animals, Base Composition, Base Sequence, Cloning, Molecular, DNA Transposable Elements, DNA Restriction Enzymes, DNA, Ribosomal, Drosophila melanogaster, Genes, Nucleic Acid Hybridization
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