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Nucleotide sequence of a macronuclear DNA molecule coding for α-tubulin from the ciliate Stylonychia lemnae. Special codon usage: TAA is not a translation termination codon
Open accessAbstract
The gene-sized macronuclear DNA of the hypotrichous ciliate Stylonychia lemnae contains two size classes of DNA molecules (1.85 and 1.73 kbp) coding for alpha-tubulin. Each macronucleus contains about 55000 copies of the 1.85 kbp molecules and about 17000 copies of the 1.73 kbp DNA molecules. Five macronuclear molecules of these sequences were cloned and sequenced, one, from the 1.85 kbp size class in its entirety. The 5 sequences fell into two classes suggesting that Stylonychia lemnae contains at least two different alpha-tubulin genes. All 5 clones show the codon TAA in the same nucleotide positions of the coding region. In this position the TAA codon cannot function as a translational stop codon and we suggest that this codon codes for the amino acid glutamine. The nucleotide sequence of the coding region as well as the encoded amino acid sequence is highly conserved compared to alpha-tubulin genes from vertebrates. The noncoding regions show several putative transcription-regulatory sequences as well as sequences presumably functioning as replication origins.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
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The guanine and cytosine content of genomic DNA and bacterial evolution.
Muto, A., & Osawa, S. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.1.166 · Cited by 578
Alessandretti, L., Aslak, U., & Lehmann, S. (2020) · Nature · doi:10.1038/s41586-020-2909-1 · Cited by 361
Identification of an acetylation site of Chlamydomonas alpha-tubulin.
LeDizet, M., & Piperno, G. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.16.5720 · Cited by 320
Codon reassignment (codon capture) in evolution
Osawa, S., & Jukes, T. H. (1989) · Journal of Molecular Evolution · doi:10.1007/bf02103422 · Cited by 289
Genetic Codes with No Dedicated Stop Codon: Context-Dependent Translation Termination
Swart, E. C., Serra, V., Petroni, G., & Nowacki, M. (2016) · Cell · doi:10.1016/j.cell.2016.06.020 · Cited by 167
Metadata
- Authors
- Helftenbem, E.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1985
- Volume
- 13
- Issue
- 2
- Pages
- 415-433
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2053285252
- Cited by
- 180
- References
- 1
- FWCI
- 10.65
- Type
- journal article
- Language
- en
- Open access
- green
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- Helftenbem, E. (1985). Nucleotide sequence of a macronuclear DNA molecule coding for α-tubulin from the ciliate Stylonychia lemnae. Special codon usage: TAA is not a translation termination codon. Nucleic Acids Research. https://doi.org/10.1093/nar/13.2.415
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