The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Removal of repeated sequences from hybridisation probes
Open accessAbstract
Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Sinclair, A. H., Berta, P., Palmer, M. S., Hawkins, J. R., Griffiths, B. L., Smith, M. J., Foster, J. W., Frischauf, A.-M., Lovell-Badge, R., & Goodfellow, P. N. (1990) · Nature · doi:10.1038/346240a0 · Cited by 3,236
Lichter, P., Cremer, T., Borden, J., Manuelidis, L., & Ward, D. C. (1988) · Human Genetics · doi:10.1007/bf01790090 · Cited by 1,160
Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg
Vidal, S. M., Malo, D., Vogan, K., Skamene, E., & Gros, P. (1993) · Cell · doi:10.1016/0092-8674(93)90135-d · Cited by 1,104
Molecular characterization of the mouse agouti locus
Bultman, S. J., Michaud, E. J., & Woychik, R. P. (1992) · Cell · doi:10.1016/s0092-8674(05)80067-4 · Cited by 801
Altered Growth of Human Colon Cancer Cell Lines Disrupted at Activated Ki- ras
Shirasawa, S., Furuse, M., Yokoyama, N., & Sasazuki, T. (1993) · Science · doi:10.1126/science.8465203 · Cited by 620
Metadata
- Authors
- Sealey, P. G., Whittaker, P. A., & Southern, E. M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1985
- Volume
- 13
- Issue
- 6
- Pages
- 1905-1922
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2074681588
- Cited by
- 283
- References
- 1
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- green
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- Sealey, P. G., Whittaker, P. A., & Southern, E. M. (1985). Removal of repeated sequences from hybridisation probes. Nucleic Acids Research. https://doi.org/10.1093/nar/13.6.1905
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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