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The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis
Open accessAbstract
A rapid and versatile method has been developed for the synthesis of oligonucleotides which contain an aliphatic amino group at their 5' terminus. This amino group reacts specifically with a variety of electrophiles, thereby allowing other chemical species to be attached to the oligonucleotide. This chemistry has been utilized to synthesize several fluorescent derivatives of an oligonucleotide primer used in DNA sequence analysis by the dideoxy (enzymatic) method. The modified primers are highly fluorescent and retain their ability to specifically prime DNA synthesis. The use of these fluorescent primers in DNA sequence analysis will enable DNA sequence analysis to be automated.
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Citations
Cited by
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
“Both of these improvements contributed to the development of increasingly automated DNA sequencing machines [27,28,29,30,31,32,33], and subsequently the fi rst crop of commercial DNA sequencing machines [34] which were used to sequence the genomes of increasingly complex species.”
BackgroundA System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides
Prober, J. M., Trainor, G. L., Dam, R. J., Hobbs, F. W., Robertson, C. W., Zagursky, R. J., Cocuzza, A. J., Jensen, M. A., & Baumeister, K. (1987) · Science · doi:10.1126/science.2443975 · Cited by 855
Metadata
- Authors
- Smith, L. M., Fung, S., Hunkapiller, M. W., Hunkapiller, T. J., & Hood, L. E.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1985
- Volume
- 13
- Issue
- 7
- Pages
- 2399-2412
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2047352138
- Cited by
- 342
- References
- 0
- FWCI
- 6.71
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis. https://app.cochat.ai/papers/10.1093/nar/13.7.2399
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- Smith, L. M., Fung, S., Hunkapiller, M. W., Hunkapiller, T. J., & Hood, L. E. (1985). The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis. Nucleic Acids Research. https://doi.org/10.1093/nar/13.7.2399
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