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Cloning and sequencing of Serratia protease gene
Open accessAbstract
The gene encoding an extracellular metalloproteinase from Serratia sp. E-15 has been cloned, and its complete nucleotide sequence determined. The amino acid sequence deduced from the nucleotide sequence reveals that the mature protein of the Serratia protease consists of 470 amino acids with a molecular weight of 50,632. The G+C content of the coding region for the mature protein is 58%; this high G+C content is due to a marked preference for G+C bases at the third position of the codons. The gene codes for a short pro-peptide preceding the mature protein. The Serratia protease gene was expressed in Escherichia coli and Serratia marcescens; the former produced the Serratia protease in the cells and the latter in the culture medium. Three zinc ligands and an active site of the Serratia protease were predicted by comparing the structure of the enzyme with those of thermolysin and Bacillus subtilis neutral protease.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Van Wart, H. E., & Birkedal-Hansen, H. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.14.5578 · Cited by 1,475
RTX proteins: a highly diverse family secreted by a common mechanism
Linhartová, I., Bumba, L., Mašín, J., Basler, M., Osička, R., Kamanová, J., Procházková, K., Adkins, I., Hejnová-Holubová, J., Sadílková, L., et al. (2010) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2010.00231.x · Cited by 530
Baumann, U., Wu, S., Flaherty, K. M., & McKay, D. B. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06009.x · Cited by 473
Burger, M., Woods, R. G., McCarthy, C., & Beacham, I. R. (2000) · Microbiology · doi:10.1099/00221287-146-12-3149 · Cited by 48
Metadata
- Authors
- Nakahama, K., Yoshimura, K., Marumoto, R., Kikuchi, M., Lee, I. S., Hase, T., & Matsubara, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1986
- Volume
- 14
- Issue
- 14
- Pages
- 5843-5855
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1987061077
- Cited by
- 191
- References
- 1
- FWCI
- 5.66
- Type
- journal article
- Language
- en
- Open access
- green
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- Nakahama, K., Yoshimura, K., Marumoto, R., Kikuchi, M., Lee, I. S., Hase, T., & Matsubara, H. (1986). Cloning and sequencing of Serratia protease gene. Nucleic Acids Research. https://doi.org/10.1093/nar/14.14.5843
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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