The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
PCR-based cDNA library construction: general cDNA libraries at the level of a few cells
Open accessAbstract
A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers complementary to oligo(dA) and oligo(dG) ends of the cDNA. For insertion of the cDNA into a vector a controlled trimming of the 3' ends of the cDNA by Klenow enzyme was used. Starting from 10 J558L micron3 myeloma cells, total cDNA was synthesized and amplified approximately 10(5) fold. A library containing 10(6) clones was established from 1/6 of the amplified cDNA. Screening of the library with probes for three genes expressed in these cells revealed a number of corresponding clones in each case. The longest obtained clones contained inserts of 1.5 kb length. No sequences originating from carriers or from rRNA was found in 14 randomly picked clones.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
Sanger, F., & Coulson, A. R. (1975) · Journal of Molecular Biology · doi:10.1016/0022-2836(75)90213-2 · Cited by 2,709
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Museum of spatial transcriptomics
Moses, L., & Pachter, L. (2022) · Nature Methods · doi:10.1038/s41592-022-01409-2 · Cited by 1,255
Museum of Spatial Transcriptomics
Moses, L., & Pachter, L. (2021) · doi:10.1101/2021.05.11.443152 · Cited by 1,255
Single-cell RNA-seq: advances and future challenges
Saliba, A.-E., Westermann, A. J., Gorski, S. A., & Vogel, J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku555 · Cited by 847
“The initial protocol stems from the late 1980s (103) and was later optimized for microarray analysis (90,104) and subsequently adapted to RNA-seq (93,105–107).”
A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations
Matthews, W., Jordan, C. T., Wiegand, G. W., Pardoll, D., & Lemischka, I. R. (1991) · Cell · doi:10.1016/0092-8674(91)90010-v · Cited by 593
Pikielny, C. W., Hasan, G., Rouyer, F., & Rosbash, M. (1994) · Neuron · doi:10.1016/0896-6273(94)90150-3 · Cited by 353
Metadata
- Authors
- Belyavsky, A., Vinogradova, T., & Rajewsky, K.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1989
- Volume
- 17
- Issue
- 8
- Pages
- 2919-2932
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2033792606
- Cited by
- 283
- References
- 3
- FWCI
- 7.81
- Type
- journal article
- Language
- en
- Open access
- bronze
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