The DOI matches its Crossref record.
Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants
Open accessAbstract
Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to methylation for these strains are presented, together with the evaluation of the most promising strains in practical recombinant cloning situations. Host strains are recommended for different recombinant cloning requirements. These data also provide a rational basis for future construction of 'ideal' hosts combining optimal methylation tolerance with additional advantageous mutations.
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Citations
Cited by
Paidhungat, M., & Garrett, S. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.11.6339 · Cited by 191
Asymmetric methylation in the hypermethylated CpG promoter region of the human L1 retrotransposon
Woodcock, D. M., Lawler, C. B., Linsenmeyer, M. E., Doherty, J. P., & Warren, W. D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.12.7810 · Cited by 168
Self-citationKrappmann, S., Bignell, E. M., Reichard, U., Rogers, T., Haynes, K., & Braus, G. H. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04015.x · Cited by 133
Deng, L., Spencer, B. L., Holmes, J. A., Mu, R., Rego, S., Weston, T. A., Hu, Y., Sanches, G. F., Yoon, S., Park, N., et al. (2019) · PLOS Pathogens · doi:10.1371/journal.ppat.1007848 · Cited by 74
Almirón, M., Martı́nez, M., Sanjuan, N., & Ugalde, R. A. (2001) · Infection and Immunity · doi:10.1128/iai.69.10.6225-6230.2001 · Cited by 54
Metadata
- Authors
- Woodcock, D. M., Crowther, P. J., Doherty, J., Jefferson, S., DeCruz, E., Noyer-Weidner, M., Smith, S. S., Michael, M. Z., & Graham, M. W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1989
- Volume
- 17
- Issue
- 9
- Pages
- 3469-3478
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2012031105
- Cited by
- 761
- References
- 0
- FWCI
- 7.33
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. https://app.cochat.ai/papers/10.1093/nar/17.9.3469
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- Woodcock, D. M., Crowther, P. J., Doherty, J., Jefferson, S., DeCruz, E., Noyer-Weidner, M., Smith, S. S., Michael, M. Z., & Graham, M. W. (1989). Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Research. https://doi.org/10.1093/nar/17.9.3469
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