The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
A sensitive method for the determination of protein-DNA binding specificities
Open accessAbstract
We describe a sensitive and rapid method for determination of the sequence specificity of DNA binding proteins. The method allows recovery of specific sites using the small amounts of protein present in crude cell extracts or produced by cell-free translation reactions. Extract proteins are incubated with a pool of random sequence oligonucleotides, complexes purified by immunoprecipitation, and bound DNA amplified by the Polymerase Chain Reaction (PCR). This DNA is then used in further rounds of binding, immunoprecipitation, and amplification, until specific binding is detectable. With the transcription factor SRF as a model system, we demonstrate that authentic high affinity binding sites are recovered, and show that epitope tagging can be used to allow recovery of sites when specific antibodies are unavailable. We also show that specific sites bound by the Fos protein, which binds DNA with high affinity only when complexed with other polypeptides, are easily recovered by this technique.
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Cites
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CpG-rich islands and the function of DNA methylation
Bird, A. P. (1986) · Nature · doi:10.1038/321209a0 · Cited by 3,777
[1] Production of single-stranded plasmid DNA
Vieira, J., & Messing, J. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)53044-0 · Cited by 2,597
Induction of c-fos-like protein in spinal cord neurons following sensory stimulation
Hunt, S. P., Pini, A., & Evan, G. (1987) · Nature · doi:10.1038/328632a0 · Cited by 1,972
Murre, C., McCaw, P. S., Vaessin, H., Caudy, M., Jan, L. Y., Jan, Y. N., Cabrera, C. V., Buskin, J. N., Hauschka, S. D., Lassar, A. B., et al. (1989) · Cell · doi:10.1016/0092-8674(89)90434-0 · Cited by 1,815
A C-terminal signal prevents secretion of luminal ER proteins
Munro, S., & Pelham, H. R. B. (1987) · Cell · doi:10.1016/0092-8674(87)90086-9 · Cited by 1,721
Fos and jun: The AP-1 connection
Curran, T., & Franza, B. R. (1988) · Cell · doi:10.1016/0092-8674(88)90024-4 · Cited by 1,567
Norman, C., Runswick, M., Pollock, R., & Treisman, R. (1988) · Cell · doi:10.1016/0092-8674(88)90244-9 · Cited by 957
Self-citationTreisman, R. (1986) · Cell · doi:10.1016/0092-8674(86)90882-2 · Cited by 847
Self-citation
Cited by
Muhlethaler-Mottet, A. (1997) · The EMBO Journal · doi:10.1093/emboj/16.10.2851 · Cited by 507
(1997) · Biological Chemistry · doi:10.1515/bchm.1997.378.10.1079 · Cited by 499
I kappa B epsilon, a novel member of the Ikappa B family, controls RelA and cRel NF-kappa B activity
Whiteside, S. T. (1997) · The EMBO Journal · doi:10.1093/emboj/16.6.1413 · Cited by 395
Definition of a consensus DNA binding site for SRY
Harley, V. R., Lovell-Badge, R., & Goodfellow, P. N. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.8.1500 · Cited by 386
HITS-CLIP: panoramic views of protein-RNA regulation in living cells
Darnell, R. B. (2010) · WIREs RNA · doi:10.1002/wrna.31 · Cited by 382
Metadata
- Authors
- Pollock, R., & Treisman, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 18
- Issue
- 21
- Pages
- 6197-6204
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2020257254
- Cited by
- 340
- References
- 41
- FWCI
- 7.16
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
