The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies
Open accessAbstract
We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C:C mismatches reduced overall PCR product yield about 100-fold, A:A mismatches about 20-fold. All other 3'-terminal mismatches were efficiently amplified, although the G:G mismatches appeared to be more sensitive to sequence context and dNTP concentrations than other mismatches. It should be noted that mismatches of T with either G, C, or T had a minimal effect on PCR product yield. Double mismatches within the last four bases of a primer-template duplex where one of the mismatches is at the 3' terminal nucleotide, in general, reduced PCR product yield dramatically. The presence of a mismatched T at the 3'-terminus, however, allowed significant amplification even when coupled with an adjacent mismatch. Furthermore, even two mismatched Ts at the 3'-terminus allowed efficient amplification.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Comparison between DNA melting thermodynamics and DNA polymerase fidelity.
Petruska, J., Goodman, M. F., Boosalis, M. S., Sowers, L. C., Cheong, C., & Tinoco, I. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.17.6252 · Cited by 316
Cited by
Quick, J., Grubaugh, N. D., Pullan, S. T., Claro, I. M., Smith, A. D., Gangavarapu, K., Oliveira, G., Robles-Sikisaka, R., Rogers, T. F., Beutler, N. A., et al. (2017) · Nature Protocols · doi:10.1038/nprot.2017.066 · Cited by 1,190
PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update
Wang, X., Spandidos, A., Wang, H., & Seed, B. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr1013 · Cited by 720
Hofreiter, M. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.23.4793 · Cited by 684
Biochemical Basis of DNA Replication Fidelity
Goodman, M. F., Creighton, S., Bloom, L. B., Petruska, J., & Kunkel, T. A. (1993) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239309086792 · Cited by 446
Stadhouders, R., Pas, S. D., Anber, J., Voermans, J., Mes, T. H. M., & Schutten, M. (2010) · The Journal of Molecular Diagnostics · doi:10.2353/jmoldx.2010.090035 · Cited by 399
Metadata
- Authors
- Kwok, S., Kellogg, D. E., McKinney, N., Spasic, D., Goda, L., Levenson, C., & Sninsky, J. J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 18
- Issue
- 4
- Pages
- 999-1005
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W1979388953
- Cited by
- 1,036
- References
- 3
- FWCI
- 18.23
- Type
- journal article
- Language
- en
- Open access
- green
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