The DOI matches its Crossref record.
Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis
Open accessAbstract
A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was used quantitatively to measure accumulated levels of RNA transcripts in total mouse RNAs derived from male germ cells at various spermatogenic stages. RNA levels for two X-linked enzymes, phosphoglycerate kinase (PGK-1) and hypoxanthine phosphoribosyl transferase (HPRT), both decrease during spermatogenesis, although the transcript levels decrease much more rapidly for PGK-1. RNA for the Y-linked ZFY (zinc finger protein) is elevated in all spermatogenic cell fractions tested, being particularly high in leptotene/zygotene spermatocytes and round spermatids. RNA for adenine phosphoribosyltransferase (APRT) increases 5-fold to a peak during late pachynema. RNA for PGK-2, undetectable in spermatogonial cells, increases at least 50-fold by the round spermatid stage. DNA (cytosine-5-)-methyltransferase (MTase) transcript levels are over an order of magnitude higher throughout spermatogenesis than in non-dividing liver cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Natural Selection and the Origin of jingwei , a Chimeric Processed Functional Gene in Drosophila
Long, M., & Langley, C. H. (1993) · Science · doi:10.1126/science.7682012 · Cited by 416
[6] Purification, culture, and fractionation of spermatogenic cells
Bellvé, A. R. (1993) · Methods in Enzymology · doi:10.1016/0076-6879(93)25009-q · Cited by 364
Self-citationKeratinocyte growth regulation in fibroblast cocultures via a double paracrine mechanism
Maas-Szabowski, N., Shimotoyodome, A., & Fusenig, N. E. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.12.1843 · Cited by 300
Zhou, R., Chen, F., Chang, F., Bai, Y., & Chen, L. (2013) · Journal of Psychiatric Research · doi:10.1016/j.jpsychires.2013.05.013 · Cited by 54
Zhu, C., Liang, M., Li, Y., Feng, X., Hong, J., & Zhou, R. (2018) · International Journal of Neuropsychopharmacology · doi:10.1093/ijnp/pyy006 · Cited by 32
Metadata
- Authors
- Singer-Sam, J., Robinson, M. O., Bellvé, A. R., Simon, M. L., & Riggs, A. D.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 18
- Issue
- 5
- Pages
- 1255-1259
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2074199894
- Cited by
- 232
- References
- 0
- FWCI
- 23.20
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis. https://app.cochat.ai/papers/10.1093/nar/18.5.1255
- The paper
- Singer-Sam, J., Robinson, M. O., Bellvé, A. R., Simon, M. L., & Riggs, A. D. (1990). Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis. Nucleic Acids Research. https://doi.org/10.1093/nar/18.5.1255
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
