The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Keratinocyte growth regulation in fibroblast cocultures via a double paracrine mechanism
Abstract
Epithelial-mesenchymal interactions play an important role in regulating tissue homeostasis and repair. For skin, the regulatory mechanisms of epidermal-dermal interactions were studied in cocultures of normal human epidermal keratinocytes (NEK) and dermal fibroblasts (HDF) rendered postmitotic by gamma-irradiation (HDFi). The expression kinetics of different cytokines and their receptors with presumed signalling function in skin were determined at the RNA and protein level in mono- and cocultured NEK and HDFi. In cocultured HDFi, mRNA and protein synthesis of keratinocyte growth factor (KGF) (FGF-7) was strongly enhanced, whereas in cocultured keratinocytes interleukin (IL)-1α and -1β mRNA expression increased compared to monocultures. Thus we postulated that IL-1, which had no effect on keratinocyte proliferation, induced in fibroblasts the expression of factors stimulating keratinocyte proliferation, such as KGF. The functional significance of this reciprocal modulation was substantiated by blocking experiments. Both IL-1α- and -1β-neutralizing antibodies and IL-1 receptor antagonist significantly reduced keratinocyte proliferation supposedly through abrogation of KGF production, because IL-1 antibodies blocked the induced KGF production. These data indicate a regulation of keratinocyte growth by a double paracrine mechanism through release of IL-1 which induces KGF in cocultured fibroblasts. Thus IL-1, in addition to its proinflammatory function in skin, may play an essential role in regulating tissue homeostasis.
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Self-citation
Cited by
Fibroblast heterogeneity: more than skin deep
Sorrell, J. M., & Caplan, A. I. (2004) · Journal of Cell Science · doi:10.1242/jcs.01005 · Cited by 553
c-Jun and JunB Antagonistically Control Cytokine-Regulated Mesenchymal–Epidermal Interaction in Skin
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Self-citationCurrent Progress in Keloid Research and Treatment
Butler, P. D., Longaker, M. T., & Yang, G. P. (2008) · Journal of the American College of Surgeons · doi:10.1016/j.jamcollsurg.2007.12.001 · Cited by 274
Fibroblast heterogeneity and its implications for engineering organotypic skin models in vitro
Sriram, G., Bigliardi, P. L., & Bigliardi-Qi, M. (2015) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2015.08.001 · Cited by 269
Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients
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Metadata
- Authors
- Maas-Szabowski, N., Shimotoyodome, A., & Fusenig, N. E.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1999-06-15
- Volume
- 112
- Issue
- 12
- Pages
- 1843-1853
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2186509953
- Cited by
- 300
- References
- 48
- FWCI
- 9.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- Maas-Szabowski, N., Shimotoyodome, A., & Fusenig, N. E. (1999). Keratinocyte growth regulation in fibroblast cocultures via a double paracrine mechanism. Journal of Cell Science. https://doi.org/10.1242/jcs.112.12.1843
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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