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Capillary gel electrophoresis for rapid, high resolution DNA sequencing
Open accessAbstract
Capillary gel electrophoresis has been demonstrated for the separation and detection of DNA sequencing samples. Enzymatic dideoxy nucleotide chain termination was employed, using fluorescently tagged oligonucleotide primers and laser based on-column detection (limit of detection is 6,000 molecules per peak). Capillary gel separations were shown to be three times faster, with better resolution (2.4 x), and higher separation efficiency (5.4 x) than a conventional automated slab gel DNA sequencing instrument. Agreement of measured values for velocity, resolution and separation efficiency with theory, predicts further improvements will result from increased electric field strengths (higher voltages and shorter capillaries). Advantages of capillary gel electrophoresis for automatic DNA sequencing instruments and for genomic sequencing are discussed.
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Cited by
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
“Both of these improvements contributed to the development of increasingly automated DNA sequencing machines [27,28,29,30,31,32,33], and subsequently the fi rst crop of commercial DNA sequencing machines [34] which were used to sequence the genomes of increasingly complex species.”
BackgroundOverview of manual and automated DNA sequencing by the dideoxy chain termination method
CHEN, E., KUANG, W., & LEE, A. (1991) · Methods · doi:10.1016/s1046-2023(05)80158-6 · Cited by 833
Contributions of PCR-Based Methods to Plant Systematics and Evolutionary Biology
Wolfe, A. D., & Liston, A. (1998) · Molecular Systematics of Plants II · doi:10.1007/978-1-4615-5419-6_2 · Cited by 411
Durney, B. C., Crihfield, C. L., & Holland, L. A. (2015) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-015-8703-5 · Cited by 92
Metadata
- Authors
- Swerdlow, H., & Gesteland, R.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 18
- Issue
- 6
- Pages
- 1415-1419
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2015988576
- Cited by
- 360
- References
- 0
- FWCI
- 28.78
- Type
- journal article
- Language
- en
- Open access
- green
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- Swerdlow, H., & Gesteland, R. (1990). Capillary gel electrophoresis for rapid, high resolution DNA sequencing. Nucleic Acids Research. https://doi.org/10.1093/nar/18.6.1415
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