The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A computer program for selection of oligonucleotide primers for polymerase chain reactions
Open accessAbstract
We have designed a computer program which rapidly scans nucleic acid sequences to select all possible pairs of oligonucleotides suitable for use as primers to direct efficient DNA amplification by the polymerase chain reaction. This program is based on a set of rules which define in generic terms both the sequence composition of the primers and the amplified region of DNA. These rules (1) enhance primer-to-target sequence hybridization avidity at critical 3'-end extension initiation sites, (2) facilitate attainment of full length extension during the 72 degrees C phase, by minimizing generation of incomplete or nonspecific product and (3) limit primer losses occurring from primer-self or primer-primer homologies. Three examples of primer sets chosen by the program that correctly amplified the target regions starting from RNA are shown. This program should facilitate the rapid selection of effective and specific primers from long gene sequences while providing a flexible choice of various primers to focus study on particular regions of interest.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences
Rose, T. M., Schultz, E. R., Henikoff, J. G., Pietrokovski, S., McCallum, C. M., & Henikoff, S. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.7.1628 · Cited by 696
General concepts for PCR primer design.
Dieffenbach, C. W., Lowe, T. M., & Dveksler, G. S. (1993) · Genome Research · doi:10.1101/gr.3.3.s30 · Cited by 484
Self-citationSanter, R., Schneppenheim, R., Dombrowski, A., Götze, H., Steinmann, B., & Schaub, J. (1997) · Nature Genetics · doi:10.1038/ng1197-324 · Cited by 335
A role for TGF-beta in oligodendrocyte differentiation.
McKinnon, R. D., Piras, G., Ida, J. A., & Dubois-Dalcq, M. (1993) · The Journal of cell biology · doi:10.1083/jcb.121.6.1397 · Cited by 248
Metadata
- Authors
- Lowe, T., Sharefkin, J., Yang, S. Q., & Dieffenbach, C. W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1990
- Volume
- 18
- Issue
- 7
- Pages
- 1757-1761
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2142448093
- Cited by
- 193
- References
- 1
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lowe, T., Sharefkin, J., Yang, S. Q., & Dieffenbach, C. W. (1990). A computer program for selection of oligonucleotide primers for polymerase chain reactions. Nucleic Acids Research. https://doi.org/10.1093/nar/18.7.1757
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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