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Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans

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Brody, Howard, Griffith, James, Cuticchia, Anthony J., Arnold, Jonathan, Timberlake, William E.·1991·Nucleic Acids Research

Abstract

Development of physical genomic maps is facilitated by identification of overlapping recombinant DNA clones containing long chromosomal DNA inserts. To simplify the analysis required to determine which clones in a genomic library overlap one another, we partitioned Aspergillus nidulans cosmid libraries into chromosome-specific subcollections. The eight A. nidulans chromosomes were resolved by pulsed field gel electrophoresis and hybridized to filter replicas of cosmid libraries. The subcollections obtained appeared to be representative of the chromosomes based on the correspondence between subcollection size and chromosome length. A sufficient number of clones was obtained in each chromosome-specific subcollection to predict the overlap and assembly of individual clones into a limited number of contiguous regions. This approach should be applicable to many organisms whose genomes can be resolved by pulsed field gel electrophoresis.

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Metadata

Authors
Brody, H., Griffith, J., Cuticchia, A. J., Arnold, J., & Timberlake, W. E.
Venue
Nucleic Acids Research
Published
1991
Volume
19
Issue
11
Pages
3105-3109
ISSN
  • 0305-1048 (ISSN-L)
  • 1362-4962
OpenAlex
W2063231382
Cited by
191
References
0
FWCI
3.42
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. https://app.cochat.ai/papers/10.1093/nar/19.11.3105
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Brody, H., Griffith, J., Cuticchia, A. J., Arnold, J., & Timberlake, W. E. (1991). Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. Nucleic Acids Research. https://doi.org/10.1093/nar/19.11.3105

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