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Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans
Open accessAbstract
Development of physical genomic maps is facilitated by identification of overlapping recombinant DNA clones containing long chromosomal DNA inserts. To simplify the analysis required to determine which clones in a genomic library overlap one another, we partitioned Aspergillus nidulans cosmid libraries into chromosome-specific subcollections. The eight A. nidulans chromosomes were resolved by pulsed field gel electrophoresis and hybridized to filter replicas of cosmid libraries. The subcollections obtained appeared to be representative of the chromosomes based on the correspondence between subcollection size and chromosome length. A sufficient number of clones was obtained in each chromosome-specific subcollection to predict the overlap and assembly of individual clones into a limited number of contiguous regions. This approach should be applicable to many organisms whose genomes can be resolved by pulsed field gel electrophoresis.
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Cited by
Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans.
Brown, D. W., Yu, J. H., Kelkar, H. S., Fernandes, M., Nesbitt, T. C., Keller, N. P., Adams, T. H., & Leonard, T. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.4.1418 · Cited by 507
Histone deacetylases in fungi: novel members, new facts
Trojer, P. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg473 · Cited by 86
Metadata
- Authors
- Brody, H., Griffith, J., Cuticchia, A. J., Arnold, J., & Timberlake, W. E.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1991
- Volume
- 19
- Issue
- 11
- Pages
- 3105-3109
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2063231382
- Cited by
- 191
- References
- 0
- FWCI
- 3.42
- Type
- journal article
- Language
- en
- Open access
- green
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- Brody, H., Griffith, J., Cuticchia, A. J., Arnold, J., & Timberlake, W. E. (1991). Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. Nucleic Acids Research. https://doi.org/10.1093/nar/19.11.3105
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