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DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting
Open accessAbstract
The RAPD (or AP-PCR) DNA fingerprinting method was used to distinguish among clinical isolates of Helicobacter pylori, a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. This method uses arbitrarily chosen oligonucleotides to prime DNA synthesis from genomic sites to which they are fortuitously matched, or almost matched. Most 10-nt primers with > or = 60% G + C yielded strain-specific arrays of up to 15 prominent fragments, as did most longer (> or = 17-nt) primers, whereas most 10-nt primers with 50% G+C did not. Each of 64 independent H. pylori isolates, 60 of which were from patients in the same hospital, was distinguishable with a single RAPD primer, which suggests a high level of DNA sequence diversity within this species. In contrast, isolates from initial and followup biopsies were indistinguishable in each of three cases tested.
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Cited by
Sullivan, D. J., Haynes, K. A., Bennett, D. E., Coleman, D. C., & Westerneng, T. J. (1995) · Microbiology · doi:10.1099/13500872-141-7-1507 · Cited by 763
Geographic distribution of vacA allelic types of Helicobacter pylori
Van Doorn, L., Figueiredo, C., Mégraud, F., Pena, S., Midolo, P., De Magalhães Queiroz, D. M., Carneiro, F., Vanderborght, B., Pegado, M. D. G. F., Sanna, R., et al. (1999) · Gastroenterology · doi:10.1016/s0016-5085(99)70065-x · Cited by 444
Helicobacter pylori evolution and phenotypic diversification in a changing host
Suerbaum, S., & Josenhans, C. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1658 · Cited by 384
Succi, M., Tremonte, P., Reale, A., Sorrentino, E., Grazia, L., Pacifico, S., & Coppola, R. (2005) · FEMS Microbiology Letters · doi:10.1016/j.femsle.2005.01.037 · Cited by 284
Laparra, J. M., Olivares, M., Gallina, O., & Sanz, Y. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030744 · Cited by 153
Metadata
- Authors
- Akopyanz, N., Bukanov, N. O., Westblom, T. U., Kresovich, S., & Berg, D. E.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1992
- Volume
- 20
- Issue
- 19
- Pages
- 5137-5142
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2080490975
- Cited by
- 752
- References
- 0
- FWCI
- 36.42
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. https://app.cochat.ai/papers/10.1093/nar/20.19.5137
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- Akopyanz, N., Bukanov, N. O., Westblom, T. U., Kresovich, S., & Berg, D. E. (1992). DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Research. https://doi.org/10.1093/nar/20.19.5137
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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