Cited by 764Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesPlant Pathogens and Fungal Diseases

Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals

Sullivan, Derek J., Haynes, Ken A., Bennett, Désirée E., Coleman, David C., Westerneng, Thea J.·1995·Microbiology

Abstract

Atypical oral Candida isolates were recovered from 60 HIV-infected and three HIV-negative individuals. These organisms were germ-tube-positive and produced abundant chlamydospores which were frequently arranged in triplets or in contiguous pairs. They belonged to C. albicans serotype A and had atypical carbohydrate assimilation profiles. Fingerprinting the genomic DNA of a selection of these organisms with the C. albicans-specific probe 27A and five separate oligonucleotides, homologous to eukaryotic microsatellite repeat sequences, demonstrated that they had a very distinct genomic organization compared to C. albicans and C. stellatoidea. This was further established by random amplified polymorphic DNA (RAPD) and karyotype analysis. Comparison of 500 bp of the V3 variable region of the large ribosomal subunit genes from nine atypical isolates and the corresponding sequences determined from C. albicans, C. stellatoidea, C. tropicalis, C. parapsilosis, C. glabrata, C. kefyr and C. krusei showed that they atypical organisms formed a homogeneous cluster (100% similarity) that was significantly different from the other Candida species analysed, but was most closely related to C. albicans and C. stellatoidea. These genetic data combined with the phenotypic characteristics of these atypical organisms strongly suggest that they constitute a novel species within the genus Candida for which the name Candida dubliniensis is proposed.

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Registry

Crossref · 10.1099/13500872-141-7-1507

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24 of 24 references verified0 citation errors · 0 not checkable

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  • Candida dubliniensis, Candida parapsilosis, Candida tropicalis, and Candida krusei have all been implicated in biofilm-associated infections.

    “The closely related species Candida dubliniensis, Candida parapsilosis, Candida tropicalis, and Candida krusei have all been implicated in biofilm-associated infections (73, 157, 187).”

    From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [187]

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Authors
Sullivan, D. J., Haynes, K. A., Bennett, D. E., Coleman, D. C., & Westerneng, T. J.
Affiliations
Trinity College Dublin (IE)
Corresponding author
Coleman, David C.
Venue
Microbiology
Published
1995-07-01
Volume
141
Issue
7
Pages
1507-1521
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
OpenAlex
W2082335793
Cited by
763
References
24
Citations by year
2026: 8 · 2025: 10 · 2024: 10 · 2023: 17 · 2022: 12 · 2021: 14 · 2020: 14 · 2019: 6 · 2018: 16 · 2017: 21 · 2016: 20 · 2015: 32 · 2014: 42 · 2013: 26 · 2012: 27
FWCI
13.16
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
Keywords
Candida dubliniensis, oral candidiasis, HIV infection, chlamydospores, germ tube formation, carbohydrate assimilation, microsatellite fingerprinting, RAPD, karyology, Candida albicans, Candida stellatoidea
MeSH terms
Acquired Immunodeficiency Syndrome, Base Sequence, Candida, Candidiasis, Oral, DNA, Fungal, DNA, Ribosomal, Humans, Karyotyping, Molecular Sequence Data, Phenotype, Phylogeny, Spores, Polymerase Chain Reaction, DNA Fingerprinting, AIDS-Related Opportunistic Infections, Carbohydrate Metabolism

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