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Cited by 378

The DOI matches its Crossref record.

DNA extraction from Pleistocene bones by a silica-based purification method

Höss, Matthias, Pääbo, Svante·1993·Nucleic Acids Research
DOI 10.1093/nar/21.16.3913
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Crossref · 10.1093/nar/21.16.3913

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  • The reduced size of DNA molecules makes it difficult to separate endogenous DNA from contaminating DNA and from co-extracted small molecules that could act as inhibitors in downstream enzymatic reactions.

    “The reduced size of the DNA molecules makes it difficult to separate endogenous DNA of interest from contaminating DNA and from co-extracted small molecules that could act as inhibitors in downstream enzymatic reactions 110,111 .”

    From Ancient DNA analysis (2021) · cites this paper as [110]

    The paper’s main text (page not given in our copy) says archaeological extracts can contain components that inhibit PCR and that contemporary DNA contamination can yield misleading results. It does not say that reduced DNA size makes endogenous DNA difficult to separate from either contaminants or small inhibitory molecules; that causal explanation is unsupported. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Citations

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Research works printed but not linked: 2

  1. H&ss.M., HanoXO. and PSSbo.S. (1993) In, MullisJC., Gibbs.R. and Ferre.F. (eds), The Polymerase Chain Reaction. Birkhfiuser, New York, in press.
  2. PSSbo,S. (1990) In, Innis.M.A., Gelfand.D.H., SninskyJ.J. and White.T.J. (eds), PCR-Protocols and Applications -A Laboratory Manual. Academic Press, San Diego.

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Metadata

Authors
Höss, M., & Pääbo, S.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
1993
Volume
21
Issue
16
Pages
3913-3914
ISSN
  • 0305-1048
  • 1362-4962
DOI
10.1093/nar/21.16.3913
Cited by
378
References
0
Language
en

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