Two regions of the Escherichia cqli 16S ribosomal RNA are important for decoding stop signals in polypeptide chain termination
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Two regions of the 16S rRNA, helix 34, and the aminoacyl site component of the decoding site at the base of helix 44, have been implicated in decoding of translational stop signals during the termination of protein synthesis. Antibiotics specific for these regions have been tested to see how they discriminate the decoding of UAA, UAG, and UGA by the two polypeptide chain release factors (RF-1 and RF-2). Spectinomycin, which interacts with helix 34, stimulated RF-1 dependent binding to the ribosome and termination. It also stimulated UGA dependent RF-2 termination at micromolar concentrations but inhibited UGA dependent RF-2 binding at higher concentrations. Alterations at position C1192 of helix 34, known to confer spectinomycin resistance, reduced the binding of f[3H]Met-tRNA to the peptidyl-tRNA site. They also impaired termination in vitro, with both factors and all three stop codons, although the effect was greater with RF-2 mediated reactions. These alterations had previously been shown to inhibit EF-G mediated translocation. As perturbations in helix 34 effect both termination and elongation reactions, these results indicate that helix 34 is close to the decoding site on the bacterial ribosome. Several antibiotics, hygromycin, neomycin and tetracycline, specific for the aminoacyl site, were shown to inhibit the binding and function of both RFs in termination with all three stop codons in vitro. These studies indicate that decoding of all stop signals is likely to occur at a similar site on the ribosome to the decoding of sense codons, the aminoacyl site, and are consistent with a location for helix 34 near this site.
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Tetracyclines prevent binding of both release factors RF-1 and 2 during termination, regardless of the stop codon.
“Tcs also prevent binding of both release factors RF-1 and 2 during termination, regardless of the stop codon (Brown et al. 1993”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Brown et al. 1993
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16S rRNA Mutation Associated with Tetracycline Resistance in a Gram-Positive Bacterium
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Trieber, C. A., Burkhardt, N., Nierhaus, K. H., & Taylor, D. E. (1998) · bchm · doi:10.1515/bchm.1998.379.7.847 · Cited by 68 · see citing works
- Authors
- Brown, C. M., McCaughan, K. K., & Tate, W. P.
- Affiliations
- University of Otago (NZ)
- Corresponding author
- Tate, Warren P.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1993
- Volume
- 21
- Issue
- 9
- Pages
- 2109-2115
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC309472
- OpenAlex
- W1966851938
- Cited by
- 42
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2019: 1 · 2017: 1 · 2014: 4 · 2012: 2
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Health Research Council of New Zealand
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Keywords
- 16S rRNA, decoding site, spectinomycin, hygromycin, neomycin, tetracycline, ribosome, Escherichia coli
- MeSH terms
- Spectinomycin, Anti-Bacterial Agents, Bacterial Proteins, Base Sequence, Cinnamates, Codon, Drug Resistance, Microbial, Escherichia coli, Hygromycin B, Molecular Sequence Data, Neomycin, Peptide Chain Termination, Translational, Peptides, Regulatory Sequences, Nucleic Acid, RNA, Ribosomal, 16S, Tetracycline
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