The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
The rate of hydrolytic deamination of 5-methylcytosine in double-stranded DNA
Open accessAbstract
The modified base, 5-methylcytosine, constitutes approximately 1% of human DNA, but sites containing 5-methylcytosine account for at least 30% of all germline and somatic point mutations. A genetic assay with a sensitivity of 1 in 10(7), based on reversion to neomycin resistance of a mutant pSV2-neo plasmid, was utilized to determine and compare the deamination rates of 5-methylcytosine and cytosine in double-stranded DNA for the first time. The rate constants for spontaneous hydrolytic deamination of 5-methylcytosine and cytosine in double-stranded DNA at 37 degrees C were 5.8 x 10(-13) s-1 and 2.6 x 10(-13) s-1, respectively. These rates are more than sufficient to explain the observed frequency of mutation at sites containing 5-methylcytosine and emphasize the importance of hydrolytic deamination as a major source of human mutations.
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Citations
Cites
Instability and decay of the primary structure of DNA
Lindahl, T. (1993) · Nature · doi:10.1038/362709a0 · Cited by 5,679
Mutagenic deamination of cytosine residues in DNA
Duncan, B. K., & Miller, J. H. (1980) · Nature · doi:10.1038/287560a0 · Cited by 792
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Repair of Endogenous DNA Damage
LINDAHL, T., & BARNES, D. E. (2000) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2000.65.127 · Cited by 714
Suppression of intestinal neoplasia by DNA hypomethylation
Laird, P. W., Jackson-Grusby, L., Fazeli, A., Dickinson, S. L., Edward Jung, W., Li, E., Weinberg, R. A., & Jaenisch, R. (1995) · Cell · doi:10.1016/0092-8674(95)90329-1 · Cited by 710
DNA methylation: past, present and future directions
Robertson, K. D., & A.Jones, P. (2000) · Carcinogenesis · doi:10.1093/carcin/21.3.461 · Cited by 607
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Do, H., & Dobrovic, A. (2015) · Clinical Chemistry · doi:10.1373/clinchem.2014.223040 · Cited by 572
Metadata
- Authors
- Shen, J.-C., Rideout, W. M., & Jones, P. A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1994
- Volume
- 22
- Issue
- 6
- Pages
- 972-976
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2073365346
- Cited by
- 457
- References
- 2
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Shen, J.-C., Rideout, W. M., & Jones, P. A. (1994). The rate of hydrolytic deamination of 5-methylcytosine in double-stranded DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/22.6.972
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