The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Cataloging altered gene expression in young and senescent cells using enhanced differential display
Open accessAbstract
Recently, a novel PCR-based technique, differential display (DD), has facilitated the study of differentially expressed genes at the mRNA level. We report here an improved version of DD, which we call Enhanced Differential Display (EDD). We have modified the technique to enhance reproducibility and to facilitate sequencing and cloning. Using EDD, we have generated and verified a catalog of genes that are differentially expressed between young and senescent human diploid fibroblasts (HDF). From 168 genetags that were identified initially, 84 could be sequenced directly from PCR amplified bands. These sequences represent 27 known genes and 37 novel genes. By Northern blot analysis we have confirmed the differential expression of a total of 23 genes (12 known, 11 novel), while 19 (seven known, 12 novel) did not show differential expression. Several of the known genes were previously observed by others to be differentially expressed between young and senescent fibroblasts, thereby validating the technique.
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Cites
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Telomeres shorten during ageing of human fibroblasts
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Cited by
TUMOR STROMA AND REGULATION OF CANCER DEVELOPMENT
Tlsty, T. D., & Coussens, L. M. (2006) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev.pathol.1.110304.100224 · Cited by 987
Microarray analysis of replicative senescence
Shelton, D. N., Chang, E., Whittier, P. S., Choi, D., & Funk, W. D. (1999) · Current Biology · doi:10.1016/s0960-9822(99)80420-5 · Cited by 764
Self-citationToussaint, O., Medrano, E. E., & von Zglinicki, T. (2000) · Experimental Gerontology · doi:10.1016/s0531-5565(00)00180-7 · Cited by 713
Replicative Senescence: Implications for in Vivo Aging and Tumor Suppression
Smith, J. R., & Pereira-Smith, O. M. (1996) · Science · doi:10.1126/science.273.5271.63 · Cited by 547
Cancer and ageing: rival demons?
Campisi, J. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1073 · Cited by 512
Metadata
- Authors
- Linskens, M. H. K., Feng, J., Andrews, W. H., Enlow, B. E., Saati, S. M., Tonkin, L. A., Funk, W. D., & Villeponteau, B.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1995
- Volume
- 23
- Issue
- 16
- Pages
- 3244-3251
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2074987564
- Cited by
- 223
- References
- 3
- FWCI
- 5.09
- Type
- journal article
- Language
- en
- Open access
- green
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- Linskens, M. H. K., Feng, J., Andrews, W. H., Enlow, B. E., Saati, S. M., Tonkin, L. A., Funk, W. D., & Villeponteau, B. (1995). Cataloging altered gene expression in young and senescent cells using enhanced differential display. Nucleic Acids Research. https://doi.org/10.1093/nar/23.16.3244
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