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A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml
Open accessAbstract
The branched DNA hybridization assay has been improved by the inclusion of the novel nucleotides, isoC and isoG, in the amplification sequences to prevent non-specific hybridization. The novel isoC, isoG-containing amplification sequences have no detectable interaction with any natural DNA sequence. The control of non-specific hybridization in turn permits increased signal amplification. Addition of a 14 site preamplifier was found to increase the signal/noise ratio 8-fold. A set of 74 oligonucleotide probes was designed to the consensus HIV POL sequence. The detection limit of this new HIV branched DNA amplifier assay was approximately 50 molecules/ml. The assay was used to measure viral load in 87 plasma samples of HIV- infected patients on triple drug therapy whose RNA titers were <500 molecules/ml. In all 11 patients viral load eventually declined to below the detection limit with the new assay.
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Citations
Cited by
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Khvorova, A., Reynolds, A., & Jayasena, S. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00801-8 · Cited by 2,645
Benner, S. A., & Sismour, A. M. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1637 · Cited by 834
Bahl, K., Senn, J. J., Yuzhakov, O., Bulychev, A., Brito, L. A., Hassett, K. J., Laska, M. E., Smith, M., Almarsson, Ö., Thompson, J., et al. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2017.03.035 · Cited by 642
Palmer, S., Wiegand, A. P., Maldarelli, F., Bazmi, H., Mican, J. M., Polis, M., Dewar, R. L., Planta, A., Liu, S., Metcalf, J. A., et al. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.10.4531-4536.2003 · Cited by 604
DNA Functional Materials Assembled from Branched DNA: Design, Synthesis, and Applications
Dong, Y., Yao, C., Zhu, Y., Yang, L., Luo, D., & Yang, D. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00294 · Cited by 533
Metadata
- Authors
- Collins, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-08-01
- Volume
- 25
- Issue
- 15
- Pages
- 2979-2984
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2120064644
- Cited by
- 317
- References
- 0
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- green
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- Collins, M. (1997). A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml. Nucleic Acids Research. https://doi.org/10.1093/nar/25.15.2979
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