The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA
Open accessAbstract
Methylation analysis of individual cytosines in genomic DNA can be determined quantitatively by bisulphite treatment and PCR amplification of the target DNA sequence, followed by restriction enzyme digestion or sequencing. Methylated and unmethylated molecules, however, have different sequences after bisulphite conversion. For some sequences this can result in bias during the PCR amplification leading to an inaccurate estimate of methylation. PCR bias is sequence dependent and often strand-specific. This study presents a simple method for detection and measurement of PCR bias for any set of primers, and investigates parameters for overcoming PCR bias.
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Cites
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
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High sensitivity mapping of methylated cytosines
Susan, J. C., Harrison, J., Paul, C. L., & Frommer, M. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.15.2990 · Cited by 1,862
Self-citationDe novo DNA cytosine methyltransferase activities in mouse embryonic stem cells
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Extensive DNA methylation spanning the Rb promoter in retinoblastoma tumors.
Stirzaker, C., Millar, D., Paul, C. L., Warnecke, P. M., Harrison, E. M., Vincent, P., Frommer, M., & Clark, S. J. (1997) · PubMed · Cited by 272
Self-citationCpNpG methylation in mammalian cells
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Cited by
DNA Methylation Analysis by Pyrosequencing
Busato, F., & Tost, J. (2014) · Encyclopedia of Metagenomics · doi:10.1007/978-1-4614-6418-1_799-1 · Cited by 499
Plagemann, A., Harder, T., Brunn, M., Harder, A., Roepke, K., Wittrock‐Staar, M., Ziska, T., Schellong, K., Rodekamp, E., Melchior, K., et al. (2009) · The Journal of Physiology · doi:10.1113/jphysiol.2009.176156 · Cited by 439
5-methylcytosine in RNA: detection, enzymatic formation and biological functions
Motorin, Y., Lyko, F., & Helm, M. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkp1117 · Cited by 386
Sensitive digital quantification of DNA methylation in clinical samples
Li, M., Chen, W.-D., Papadopoulos, N., Goodman, S. N., Bjerregaard, N. C., Laurberg, S., Levin, B., Juhl, H., Arber, N., Moinova, H., et al. (2009) · Nature Biotechnology · doi:10.1038/nbt.1559 · Cited by 341
CTCF maintains differential methylation at the Igf2/H19 locus
Schoenherr, C. J., Levorse, J. M., & Tilghman, S. M. (2003) · Nature Genetics · doi:10.1038/ng1057 · Cited by 320
Metadata
- Authors
- Warnecke, P. M., Stirzaker, C., Melki, J. R., Millar, D. S., Paul, C. L., & Clark, S. J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-11-01
- Volume
- 25
- Issue
- 21
- Pages
- 4422-4426
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2135157239
- Cited by
- 388
- References
- 8
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- green
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- Warnecke, P. M., Stirzaker, C., Melki, J. R., Millar, D. S., Paul, C. L., & Clark, S. J. (1997). Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/25.21.4422
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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