The DOI matches its Crossref record; 100 of the 100 references it cites were found in a registry or an index.
5-methylcytosine in RNA: detection, enzymatic formation and biological functions
Open accessAbstract
The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modified nucleobase in RNA still remain to be elucidated. The recent development of a bisulfite sequencing approach for efficient m(5)C localization in various RNA molecules puts ribo-m(5)C in a highly privileged position as one of the few RNA modifications whose detection is amenable to PCR-based amplification and sequencing methods. Additional progress in the field also includes the characterization of several specific RNA methyltransferase enzymes in various organisms, and the discovery of a new and unexpected link between DNA and RNA m(5)C-methylation. Numerous putative RNA:m(5)C-MTases have now been identified and are awaiting characterization, including the identification of their RNA substrates and their related cellular functions. In order to bring these recent exciting developments into perspective, this review provides an ordered overview of the detection methods for RNA methylation, of the biochemistry, enzymology and molecular biology of the corresponding modification enzymes, and discusses perspectives for the emerging biological functions of these enzymes.
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Cites
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EUKARYOTIC CYTOSINE METHYLTRANSFERASES
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Cited by
Morris, K. V., & Mattick, J. S. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3722 · Cited by 1,371
RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
Schaefer, M., Pollex, T., Hanna, K., Tuorto, F., Meusburger, M., Helm, M., & Lyko, F. (2010) · Genes & Development · doi:10.1101/gad.586710 · Cited by 746
Self-citationRNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesis
Tuorto, F., Liebers, R., Musch, T., Schaefer, M., Hofmann, S., Kellner, S., Frye, M., Helm, M., Stoecklin, G., & Lyko, F. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2357 · Cited by 631
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Self-citationChen, Z., Zhao, P., Li, F., Marquez-Lago, T. T., Leier, A., Revote, J., Zhu, Y., Powell, D. R., Akutsu, T., Webb, G. I., et al. (2020) · Briefings in Bioinformatics · doi:10.1093/bib/bbz041 · Cited by 417
Metadata
- Authors
- Motorin, Y., Lyko, F., & Helm, M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-12-08
- Volume
- 38
- Issue
- 5
- Pages
- 1415-1430
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2105938928
- Cited by
- 386
- References
- 100
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- gold
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